Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
high resolution crystal structures of both human NATs, including NAT1 in complex with the irreversible inhibitor 2-bromoacetanilide, a site-directed NAT1 mutant, NAT1-F125S, and a NAT2-CoA complex are presented. By comparing the structures with known prokaryotic structures, evidences are provided for novel structural features of human NATs that are absent in bacterial enzymes, including an insertion that produces a significant difference in the structure of the carboxyl terminus of the eukaryotic enzymes | Homo sapiens |
high resolution crystal structures of both human NATs, including NAT1 in complex with the irreversible inhibitor 2-bromoacetanilide, a site-directed NAT1 mutant, NAT1-F125S, and a NAT2-CoA complex are presented. By comparing the structures with known prokaryotic structures, evidences are provided for novel structural features of human NATs that are absent in bacterial enzymes, including an insertion that produces a significant difference in the structure of the carboxyl terminus of the eukaryotic enzymes. A complete picture of the distinct substrate selectivity of human NAT1 and NAT2, as well as key features of the naturally occurring variants of NAT1 and NAT2 is provided | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F125S | high resolution crystal structure of mutant F125S is generated | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-bromoacetanilide | irreversible inhibitor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
arylamine N-acetyltransferase 1 | - |
Homo sapiens |
NAT1 | - |
Homo sapiens |
NAT2 | - |
Homo sapiens |