Activating Compound | Comment | Organism | Structure |
---|---|---|---|
membrane transporter | for acetyl-CoA and acetyltransferase from cytosol into lumen of the ER/ER Golgi intermediate compartment | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | pathogenesis of late-onset Alzheimer disease, post-translational regulation of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels, a membrane protein that acts as the rate-limiting enzyme in the generation of Alzheimer disease amyloid beta-peptide, acetylation protect the protein from degradation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
PCR-amplification, stable transfection of CHO cells, with myc-tag, overexpressing enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | silencing of enzyme gene | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) | Homo sapiens | 5789 | - |
Golgi membrane | catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) | Homo sapiens | 139 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
SDS-PAGE, rough estimation from figure | Homo sapiens |
28000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 | Homo sapiens | - |
CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
H4 neuroglioma cell | - |
Homo sapiens | - |
SH-SY5Y cell | neuroblastoma | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 | - |
Homo sapiens | CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1 | - |
? | |
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 | 50 mM Tris-HCl, pH 8.0, 30°C | Homo sapiens | CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1 | - |
? | |
acetyl-CoA + histone H3 N-terminal tail | 50 mM Tris-HCl, pH 8.0, 30°C | Homo sapiens | CoA + acetylated histone H3 N-terminal tail | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATase1 | - |
Homo sapiens |
ATase2 | - |
Homo sapiens |
lysine acetyltransferase | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | transfected cells increase acetyltransferase activity 2fold, and by the treatment with the lipid second messenger ceramide | up |
Homo sapiens | transfected cells increase acetyltransferase activity 3fold, and by the treatment with the lipid second messenger ceramide | up |
General Information | Comment | Organism |
---|---|---|
metabolism | beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC) | Homo sapiens |