Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphorylation of histone H3 enhances Gcn5 enzyme activity | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of Gcn5 protein catalytic domain in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D214A | site-directed mutagenesis, increased turnover | Saccharomyces cerevisiae |
E173Q | site-directed mutagenesis, 500-600 decrease in turnover, no effect on substrate binding, Glu173 is the general base catalyst | Saccharomyces cerevisiae |
H145A | site-directed mutagenesis, altered peptide binding | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Gcn5 protein mutants | Saccharomyces cerevisiae | |
0.49 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
three-dimensional structure of the ternary complex of tGcn5 domain | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene Gcn5 | - |
Purification (Comment) | Organism |
---|---|
recombinant catalytic domain of Gcn5 protein | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone | - |
Saccharomyces cerevisiae | CoA + acetylhistone | - |
? | |
acetyl-CoA + histone H3 | Gcn5 protein: specific for Lys14 of histone H3 | Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3-peptide | - |
? | |
acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 -peptide | - |
? | |
additional information | Gcn5 and PCAF protein are transcription cofactors | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAT | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Gcn5 protein mutants | Saccharomyces cerevisiae | |
0.0283 | - |
acetyl-CoA | Gcn5 protein | Saccharomyces cerevisiae | |
0.0283 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |