Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slightly activates | Bos taurus | |
acetic acid anhydride | activates | Bos taurus | |
dithiothreitol | slightly activates | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | concentration-dependent | Bos taurus | |
Cu2+ | - |
Bos taurus | |
diethyl dicarbonate | inhibits intramolecular migration of the acetyl group from position 7 to 9 | Bos taurus | |
methylamine | slightly | Bos taurus | |
N-Acetylimidazole | - |
Bos taurus | |
N-bromosuccinimide | slightly | Bos taurus | |
p-chloromercuribenzoate | - |
Bos taurus | |
Triton X-100 | above 0.02% | Bos taurus | |
Zn2+ | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00163 | - |
acetyl-CoA | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi vesicle | - |
Bos taurus | - |
- |
microsome | - |
Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + sialic acid | Bos taurus | - |
CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O(or 9-O)-acetylneuraminate | 7-O-acetyl groups can migrate to the 9-position | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
submandibular gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
subcellular distribution | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-acetylneuraminate | glycosidically bound N-acetylneuraminate | Bos taurus | CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | O-acetylation at position 7, 9-O-acetylated product appears after migration of the acetyl group from position 7 to 9 | ? | |
acetyl-CoA + sialic acid | - |
Bos taurus | CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | - |
? | |
additional information | reaction affords the transport of the acetyl group by a translocase across the Golgi membrane, followed by the enzymatic transfer of the acetyl group onto sialic acid in the Golgi lumen | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0112 | - |
CoA | - |
Bos taurus |