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Literature summary for 2.3.1.307 extracted from

  • Kawai, S.; Sugaya, Y.; Hagihara, R.; Tomita, H.; Katsuyama, Y.; Ohnishi, Y.
    Complete biosynthetic pathway of alazopeptin, a tripeptide consisting of two molecules of 6-diazo-5-oxo-L-norleucine and one Molecule of alanine (2021), Angew. Chem. Int. Ed. Engl., 60, 10319-10325 .
    View publication on PubMed
Search for more literature for 2.3.1.307

Cloned(Commentary)

Cloned (Comment) Organism
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 Kitasatospora azatica

Protein Variants

Protein Variants Comment Organism
synthesis heterologous production of N-acetyl-6-diazo-5-oxo-l-norleucine in Streptomyces albus by expression of the corresponding enzymes Kitasatospora azatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 6-diazo-5-oxo-L-norleucine Kitasatospora azatica
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 CoA + N-acetyl-6-diazo-5-oxo-L-norleucine
-
 ?
additional information Kitasatospora azatica enzyme also uses propionyl-CoA and butyryl-CoA as acyl donors, acetyl-CoA is clearly preferred. No substrates: L-lysine, 5-hydroxy-L-lysine, 5-oxo-L-lysine ?
-
-

Organism

Organism UniProt Comment Textmining
Kitasatospora azatica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 6-diazo-5-oxo-L-norleucine
-
 Kitasatospora azatica CoA + N-acetyl-6-diazo-5-oxo-L-norleucine
-
 ?
additional information enzyme also uses propionyl-CoA and butyryl-CoA as acyl donors, acetyl-CoA is clearly preferred. No substrates: L-lysine, 5-hydroxy-L-lysine, 5-oxo-L-lysine Kitasatospora azatica ?
-
-

Synonyms

Synonyms Comment Organism
azpI
-
 Kitasatospora azatica

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the tripeptide alazopeptin, but inactivation of AzpI does not affect the production of alazopeptin Kitasatospora azatica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
178.3
-
 6-diazo-5-oxo-L-norleucine pH not specified in the publication, temperature not specified in the publication Kitasatospora azatica