Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue cells | Pseudomonas sp. YGJ3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diacetylphloroglucinol | 2,4-diacetylphloroglucinol inhibits the forward reaction competitively | Pseudomonas sp. YGJ3 | |
Cl- | inhibited by 2 mM Cl- | Pseudomonas sp. YGJ3 | |
diethylpyrocarbonate | 88% inhibition at 1 mM | Pseudomonas sp. YGJ3 | |
Hg2+ | complete inhibition at 0.1 mM | Pseudomonas sp. YGJ3 | |
additional information | NaCl, MgCl2, CaCl2,FeCl3, ZnCl2, CuCl2, and NiCl2, and various metal chelators (0.5 mM), including 1,10-phenanthroline, 8-hydroxyquinoline, 2,2'-dipyridine, and catechol-3,5-disulfonic acid disodium salt, have almost no effect on enzyme activity. Neither activation nor inhibition is found with thiols (1mM) such as L-cysteine, 2-mercaptoethanol, and dithiothreitol; not inhibited by 2 mM Br- or 2 mM I-, as well as phloroglucinol, acetyl-CoA, and malonyl-CoA | Pseudomonas sp. YGJ3 | |
p-chloromercuribenzoate | complete inhibition at 1 mM | Pseudomonas sp. YGJ3 | |
pyoluteorin | inhibited by 0.05 mM pyoluteorin | Pseudomonas sp. YGJ3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
2,4-diacetylphloroglucinol | at pH 7.0 and 25°C | Pseudomonas sp. YGJ3 | |
0.26 | - |
2-acetylphloroglucinol | at pH 7.0 and 25°C | Pseudomonas sp. YGJ3 | |
0.77 | - |
resorcinol | at pH 7.0 and 25°C | Pseudomonas sp. YGJ3 | |
1.31 | - |
phloroglucinol | at pH 7.0 and 25°C | Pseudomonas sp. YGJ3 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
330000 | - |
gel filtration | Pseudomonas sp. YGJ3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2-acetylphloroglucinol | Pseudomonas sp. YGJ3 | - |
2,4-diacetylphloroglucinol + phloroglucinol | - |
r | |
2,4-diacetylphloroglucinol + phloroglucinol | Pseudomonas sp. YGJ3 | - |
2 2-acetylphloroglucinol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. YGJ3 | F7J5Y3 | subunit PhlB | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Pseudomonas sp. YGJ3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2-acetylphloroglucinol | - |
Pseudomonas sp. YGJ3 | 2,4-diacetylphloroglucinol + phloroglucinol | - |
r | |
2,4-diacetylphloroglucinol + phloroglucinol | - |
Pseudomonas sp. YGJ3 | 2 2-acetylphloroglucinol | - |
r | |
2,4-diacetylphloroglucinol + resorcinol | - |
Pseudomonas sp. YGJ3 | ? | - |
r | |
additional information | no activity with pyrocatechol | Pseudomonas sp. YGJ3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | x * 42500 + x * 38500 + x * 16600, calculated from amino acid sequence | Pseudomonas sp. YGJ3 |
heterotrimer | x * 43000 + x * 39000 + x * 17000, SDS-PAGE | Pseudomonas sp. YGJ3 |
Synonyms | Comment | Organism |
---|---|---|
MAPG acetyltransferase | - |
Pseudomonas sp. YGJ3 |
MAPG Atase | - |
Pseudomonas sp. YGJ3 |
monoacetylphloroglucinol acetyltransferase | - |
Pseudomonas sp. YGJ3 |
PhlA | subunit | Pseudomonas sp. YGJ3 |
phlACB | - |
Pseudomonas sp. YGJ3 |
phlB | subunit | Pseudomonas sp. YGJ3 |
PhlC | subunit | Pseudomonas sp. YGJ3 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | the enzyme retains its activity below 40°C for 20 min, but is inactivated at above 65°C | Pseudomonas sp. YGJ3 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 7.2 | the forward and the reverse reactions show optimum pH values at about 6.3-7.2 | Pseudomonas sp. YGJ3 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
2,4-diacetylphloroglucinol | at pH 7.0 and 25°C | Pseudomonas sp. YGJ3 |
Organism | Comment | Expression |
---|---|---|
Pseudomonas sp. YGJ3 | 2 mM Cl- and 0.05 mM pyoluteorin repress the expression of the enzyme | down |