Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA-S-acetyl-ARYFRR | linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6) | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome] | Salmonella enterica subsp. enterica serovar Typhimurium | - |
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZJW4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni HiTrap column chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome] | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RimI | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
CoA-S-acetyl-ARYFRR | at pH 7.3 and 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |