Activating Compound | Comment | Organism | Structure |
---|---|---|---|
25-hydroxycholesterol | activates | Rattus norvegicus | |
cholate | increase of activity in cholate-fed animals | Rattus norvegicus | |
cholesterol | increase of activity in cholesterol-fed animals | Rattus norvegicus | |
Mevalonolactone | intragastric administration, increasing sterol biosynthesis and increasing enzyme activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
butanol | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
Detergents | above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 | Rattus norvegicus | |
Digitonin | - |
Rattus norvegicus | |
ethanol | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
Filipin | - |
Rattus norvegicus | |
oleoyl-CoA | inhibits at high concentrations, the inhibition is prevented by serum albumin | Rattus norvegicus | |
organic solvents | - |
Rattus norvegicus | |
progesterone | - |
Rattus norvegicus | |
SC-31769 | 20-oxa analog of 7-ketosterol | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | little activity | Rattus norvegicus | 5829 | - |
endoplasmic reticulum | rough, membranes | Rattus norvegicus | 5783 | - |
microsome | intrinsic membrane enzyme, highest activity | Rattus norvegicus | - |
- |
mitochondrion | little activity | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus male Sprague-Dawley | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | CoA + cholesteryl long-chain fatty acyl ester | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Sprague-Dawley strain | - |
Rattus norvegicus male Sprague-Dawley | - |
male Sprague-Dawley strain | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
overview: effect of fasting, feeding with cholesterol or cholate, atherogenic diet administration of Triton WR 1339 and administration of mevanolactone, evidence for a circadian rhythm | Rattus norvegicus |
Storage Stability | Organism |
---|---|
frozen, stable for up to 2 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Rattus norvegicus male Sprague-Dawley | CoA + cholesterol ester | - |
? | |
long-chain fatty acyl-CoA + cholesterol | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | Rattus norvegicus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
long-chain fatty acyl-CoA + cholesterol | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | Rattus norvegicus male Sprague-Dawley | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
oleoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | - |
Rattus norvegicus male Sprague-Dawley | CoA + cholesteryl oleate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, complete loss of activity | Rattus norvegicus |