Literature summary for 2.3.1.255 extracted from

Deng, S.; McTiernan, N.; Wei, X.; Arnesen, T.; Marmorstein, R.
Molecular basis for N-terminal acetylation by human NatE and its modulation by HYPK (2020), Nat. Commun., 11, 818 .

Search for more literature for 2.3.1.255

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of N-terminally His-tagged NatA in Spodoptera frugiperda Sf9 cells, coexpression of tagged HYPK	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L814P	site-directed mutagenesis, the hNAA15 mutant is defective for HYPK inhibition and reduces hNatA thermostability, hNAA10 binding is not affected. The hNAA15-L814P-V5 hNatA complex shows an increased catalytic activity compared to wild-type hNatA	Homo sapiens
T406Y	site-directed mutagenesis, the hNAA15-T406Y-V5 hNatA mutant complex displays a decreased catalytic activity toward the hNatA substrate SESS compared to wild-type hNatA. the hNAA15 mutant can disassociate hNAA50 from hNatA in vitro, hNAA10 binding is not affected	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
hNAA50	UniProt ID Q9GZZ1, HYPK and hNAA50 can bind to hNatA simultaneously to form a tetrameric hNatE/HYPK complex. NAA50 and HYPK exhibit negative cooperative binding to NAA15 in vitro and in human cells by inducing NAA15 shifts in opposing directions. hNAA50 and HYPK inhibit hNatA activity, and HYPK is dominant	Homo sapiens
HYPK	UniProt ID Q9NX55, a protein with intrinsic NAA10 catalytic subunit inhibitory activity. HYPK and hNAA50 can bind to hNatA simultaneously to form a tetrameric hNatE/HYPK complex. NAA50 and HYPK exhibit negative cooperative binding to NAA15 in vitro and in human cells by inducing NAA15 shifts in opposing directions. hNAA50 and HYPK inhibit hNatA activity, and HYPK is dominant	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P41227 AND Q9BXJ9	NatA subunits Naa10 and Naa15	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged hNatA by affinity chromatography and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + N-terminal L-seryl-[ESSSKSRWGRPVGRRRRPVRVYP]	substrate SESS	Homo sapiens	CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[ESSSKSRWGRPVGRRRRPVRVYP]	-	?

Synonyms

Synonyms	Comment	Organism
N-terminal acetyltransferase A	-	Homo sapiens
NAA10	-	Homo sapiens
NAA15	-	Homo sapiens
NatA	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	HYPK is a negative regulator for hNatA acetylation activity	Homo sapiens
physiological function	hNatA significantly enhances the catalytic efficiency of hNatE (EC 2.3.1.258). The hNatE complex comprises subunits Naa10 and Naa15 (NatA) and Naa50. HYPK binding to hNatE largely nullifies this effect	Homo sapiens

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Release 2023.1 (January 2023)