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Literature summary for 2.3.1.255 extracted from

  • Arnesen, T.; Starheim, K.K.; Van Damme, P.; Evjenth, R.; Dinh, H.; Betts, M.J.; Ryningen, A.; Vandekerckhove, J.; Gevaert, K.; Anderson, D.
    The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation (2010), Mol. Cell. Biol., 30, 1898-1909.
    View publication on PubMed
Search for more literature for 2.3.1.255

Cloned(Commentary)

Cloned (Comment) Organism
cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
ribosome the HYPK-hNatA interaction may occur at the ribosome, active translation is not obligatory for the interaction to occur. Thus, HYPK stably associates with hNatA, common binding to the ribosomes Homo sapiens 5840
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + PCNP protein Homo sapiens i.e. PEST proteolytic signal-containing nuclear protein CoA + Nalpha-acetyl-PCNP protein
-
 ?
acetyl-CoA + peptide Homo sapiens
-
 CoA + Nalpha-acetylpeptide
-
 ?
additional information Homo sapiens endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + ACTH peptide 17 amino acids are identical to the adrenocorticotropin (ACTH) peptide sequence, the ACTH-derived lysines are replaced by arginines to minimize any potential interference by Nalpha-acetylation Homo sapiens CoA + ?
-
 ?
acetyl-CoA + PCNP protein i.e. PEST proteolytic signal-containing nuclear protein Homo sapiens CoA + Nalpha-acetyl-PCNP protein
-
 ?
acetyl-CoA + peptide
-
 Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
acetyl-CoA + SESSSKSRWGRPVGRRRRPVRVYP high-mobility-group protein A1 sequence Homo sapiens CoA + Ac-SESSSKSRWGRPVGRRRRPVRVYP
-
 ?
additional information endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
NatA
-
 Homo sapiens
NatA protein Nalpha-terminal-acetyltransferase complex
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens