Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of phosphatidylserine-liposomes increases the enzyme activity by 6.7%. Phospholipid-containing liposomes are potent stimulators of the enzyme, best is phosphatidylserine in liposomes, suggesting an important role of the net negative charges, overview | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
mitochondrial membrane | - |
Bos taurus | 31966 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sphingosine + palmitoyl-CoA | Bos taurus | - |
N-palmitoyl-D-sphingosine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | A0A3Q1MJ30 | - |
- |
Purification (Comment) | Organism |
---|---|
solubilization of membrane-bound enzyme from brain mitochondria by 2% of Trinton X-100, followed by ammonium sulfate fractionation and dialysis | Bos taurus |
Renatured (Comment) | Organism |
---|---|
reconstitution of solubilized membrane enzyme in membrane lipid liposomes. The ability of phospholipids to enhance the activity of solubilized ceramide synthase is specific and structure-related. The most potent stimulator is phosphatidylserine in liposomes, suggesting an important role of the net negative charges | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0011 | - |
partially purified enzyme, pH 7.4, 37°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sphingosine + palmitoyl-CoA | - |
Bos taurus | N-palmitoyl-D-sphingosine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase | - |
Bos taurus |
ceramide synthase 6 | - |
Bos taurus |
fatty acyl-CoA:sphingosine acyltransferase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | over 50% ox maximal activity at pH 6.5-8.0, inactive at pH 4.5, maximally active at pH 7.5 | Bos taurus |