Cloned (Comment) | Organism |
---|---|
4-hydroxy-2(1H)-quinolone is produced by Escherichia coli coexpressing PqsA and PqsD | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
malonyl-[acyl-carrier protein] | pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.035 | - |
2-aminobenzoyl-CoA | pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.104 | - |
malonyl-CoA | pH 7.5, 37°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 2-aminobenzoyl-CoA | Pseudomonas aeruginosa | the enzyme is involved in the biosynthesis of 2,4-dihydroxyquinoline. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is a highly interactive organism secreting a variety of chemical signals. One intercellular signaling system unique to Pseudomonas and Burkholderia species is the production of hydrophobic quinolines. These quinoline derivatives are 4-hydroxy-2-heptylquinoline, 3,4-dihydroxy-2-heptylquinoline, and 2,4-dihydroxyquinoline | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
pH 7.5, 37°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 2-aminobenzoyl-CoA | the enzyme is involved in the biosynthesis of 2,4-dihydroxyquinoline. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is a highly interactive organism secreting a variety of chemical signals. One intercellular signaling system unique to Pseudomonas and Burkholderia species is the production of hydrophobic quinolines. These quinoline derivatives are 4-hydroxy-2-heptylquinoline, 3,4-dihydroxy-2-heptylquinoline, and 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
malonyl-CoA + 2-aminobenzoyl-CoA | first, the 2-aminobenzoyl moiety is transferred to the active-site Cys of PqsD to form a 2-aminobenzoyl-PqsD intermediate, which then condenses with either malonyl-CoA or malonyl-acyl carrier protein to produce 3-(2-aminophenyl)-3-oxopropanoyl-CoA. This short-lived intermediate undergoes an intramolecular rearrangement to form 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
malonyl-[acyl-carrier protein] + 2-aminobenzoyl-CoA | first, the 2-aminobenzoyl moiety is transferred to the active-site Cys of PqsD to form a 2-aminobenzoyl-PqsD intermediate, which then condenses with either malonyl-CoA or malonyl-acyl carrier protein to produce 3-(2-aminophenyl)-3-oxopropanoyl-CoA. This short-lived intermediate undergoes an intramolecular rearrangement to form 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | CoA + acyl-carrier protein + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
additional information | no activity with malonic acid or 2-hydroxybenzoate | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,4-dihydroxyquinoline synthase | - |
Pseudomonas aeruginosa |
PqsD | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | synthesis of 2,4-dihydroxyquinoline, an extracellular metabolite produced by Pseudomonas aeruginosa and secreted into growth medium in stationary phase. The enzyme is essential for Pseudomonas quinolone signal synthesis | Pseudomonas aeruginosa |