Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
S317F | inactive mutant enzyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.875 | - |
2-aminobenzoyl-CoA | pH 7, 37°C | Pseudomonas aeruginosa | |
1.3 | - |
3-oxodecanoate | pH 7, 37°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 2-aminobenzoyl-CoA | Pseudomonas aeruginosa | 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. The enzyme is involved in the biosynthesis of the Pseudomonas Quinolone Signal | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxodecanoate + 2-aminobenzoyl-CoA | - |
Pseudomonas aeruginosa | CoA + 2-heptyl-4-hydroxyquinoline + CO2 + H2O | - |
? | |
malonyl-CoA + 2-aminobenzoyl-CoA | 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. The enzyme is involved in the biosynthesis of the Pseudomonas Quinolone Signal | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
malonyl-CoA + 2-aminobenzoyl-CoA | 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. Ping-pong mechanism for PqsD with 2-aminobenzoyl-CoA as first substrate. Trajectory analysis of different PqsD complexes evidences ligand-dependent induced-fit motions affecting the modified 2-aminobenzoyl-CoA funnel access to the exposure of a secondary channel. A tunnel-network is formed in which Ser317 plays an important role by binding to both substrates | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0165 | - |
2-aminobenzoyl-CoA | pH 7, 37°C | Pseudomonas aeruginosa | |
0.0165 | - |
3-oxodecanoate | pH 7, 37°C | Pseudomonas aeruginosa |