BRENDA - Enzyme Database
show all sequences of 2.3.1.203

Structure and active site residues of PglD, an N-acetyltransferase from the bacillosamine synthetic pathway required for N-glycan synthesis in Campylobacter jejuni

Rangarajan, E.S.; Ruane, K.M.; Sulea, T.; Watson, D.C.; Proteau, A.; Leclerc, S.; Cygler, M.; Matte, A.; Young, N.M.; Biochemistry 47, 1827-1836 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli AD202 cells
Campylobacter jejuni
Crystallization (Commentary)
Crystallization (Commentary)
Organism
apoenzyme or in complex with CoA, hanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.5) and 1.9 M ammonium sulfate, at 30C
Campylobacter jejuni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22025
-
2 * 22025, electrospray ionization mass spectrometry
Campylobacter jejuni
62700
-
gel filtration
Campylobacter jejuni
Organism
Organism
UniProt
Commentary
Textmining
Campylobacter jejuni
Q0P9D1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
HisTrap column chromatography and Superdex 200 gel filtration
Campylobacter jejuni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose
-
718849
Campylobacter jejuni
CoA + UDP-N,N'-diacetylbacillosamine
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotrimer
2 * 22025, electrospray ionization mass spectrometry
Campylobacter jejuni
Synonyms
Synonyms
Commentary
Organism
Cj1123c
-
Campylobacter jejuni
PglD
-
Campylobacter jejuni
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli AD202 cells
Campylobacter jejuni
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apoenzyme or in complex with CoA, hanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.5) and 1.9 M ammonium sulfate, at 30C
Campylobacter jejuni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22025
-
2 * 22025, electrospray ionization mass spectrometry
Campylobacter jejuni
62700
-
gel filtration
Campylobacter jejuni
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap column chromatography and Superdex 200 gel filtration
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose
-
718849
Campylobacter jejuni
CoA + UDP-N,N'-diacetylbacillosamine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
2 * 22025, electrospray ionization mass spectrometry
Campylobacter jejuni
Other publictions for EC 2.3.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736434
Morrison
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Acinetobacter baumannii, Acinetobacter baumannii AYE, Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
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-
-
2
7
-
-
9
-
-
-
-
-
4
-
-
-
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-
-
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-
4
-
3
-
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9
-
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-
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-
2
7
-
-
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9
-
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-
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-
-
-
-
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
718901
Hartley
Biochemical characterization o ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae MS11
Biochemistry
50
4936-4948
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-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
-
-
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-
1
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1
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1
1
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1
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1
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-
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-
-
-
-
-
-
-
-
720459
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Identification and quantificat ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Mol. Cell. Proteomics
8
2170-2185
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
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2
-
1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718849
Rangarajan
Structure and active site resi ...
Campylobacter jejuni
Biochemistry
47
1827-1836
2008
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
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-
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-
1
-
1
-
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-
-
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2
-
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-
1
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714160
Olivier
In vitro biosynthesis of UDP-N ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Biochemistry
45
13659-13669
2006
-
-
1
-
-
-
-
1
-
-
1
-
-
14
-
-
1
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
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1
-
-
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1
-
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2
1
-
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-
1
-
-
-
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1
1
-
1
1