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Literature summary for 2.3.1.201 extracted from

  • Westman, E.L.; Preston, A.; Field, R.A.; Lam, J.S.
    Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters (2008), J. Bacteriol., 190, 6060-6069.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.201

Cloned(Commentary)

Cloned (Comment) Organism
gene wbpD, expression and subcloning of His6-tagged WbpD in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of a gene wbpD knockout strain, functional complementation by expression of WbpB from Bordetella pertussis, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Pseudomonas aeruginosa
-
 CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate
-
 ?
additional information Pseudomonas aeruginosa the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene wbpD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged WbpD from Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate
-
 Pseudomonas aeruginosa CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate
-
 ?
additional information the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
WbpD
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Pseudomonas aeruginosa