Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 45 mM, 10% increase in activity | Acinetobacter baylyi ADP1 |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes, decrease in activity with buffers such as bis-Tris, HEPES, morpholinoethanesulfonic acid | Acinetobacter baylyi ADP1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 | |
CoA | above 50 mM, about 70% inhibition above 300 mM | Acinetobacter baylyi ADP1 | |
MgCl2 | 5 mM, 17% inhibition | Acinetobacter baylyi ADP1 | |
MnCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lipid particle | - |
Acinetobacter baylyi ADP1 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38100 | - |
2 * 38100, gel filtration | Acinetobacter baylyi ADP1 |
94100 | - |
gel filtration | Acinetobacter baylyi ADP1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi ADP1 | Q8GGG1 | expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Acinetobacter baylyi ADP1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-decanol | at 48% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + 2-cyclohexylethanol | at 45% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + 2-decanol | at 39% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + 4-decanol | at 15.6% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + cyclododecanol | at 80% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + cyclohexandiol | at 4.1% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + cyclohexanol | at 32% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + cyclohexanone oxime | at 5.2% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + hexadecanol | - |
Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + phenol | at 4.1% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
acyl-CoA + phenylethanol | at 99% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
additional information | enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position | Acinetobacter baylyi ADP1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38100, gel filtration | Acinetobacter baylyi ADP1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Acinetobacter baylyi ADP1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | - |
Acinetobacter baylyi ADP1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
phosphate buffer | Acinetobacter baylyi ADP1 |