Literature summary for 2.3.1.198 extracted from

Fawke, S.; Torode, T.A.; Gogleva, A.; Fich, E.A.; S?rensen, I.; Yunusov, T.; Rose, J.K.C.; Schornack, S.
Glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis (2019), New Phytol., 223, 1547-1559 .

Search for more literature for 2.3.1.198

Cloned(Commentary)

Cloned (Comment)	Organism
gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis	Solanum lycopersicum
gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis	Nicotiana benthamiana

Protein Variants

Protein Variants	Comment	Organism
additional information	gene GPAT6 knockout phenotype, overview	Nicotiana benthamiana
additional information	leaves of 6-wk-old tomato cv. Micro-Tom or gpat6-a mutant plants are subjected to a detached leaf infection assay, gpat6-a tomato fruits have increased cuticle permeability, gpat6-a tomato leaves have fewer stomata and show an increased rate of water loss compared with the wild-type. Mutant gpat6-a leaf epidermal cells possess a thicker cell wall. The gpat6-a leaf transcriptome reflects changes in cuticle and cell wall processes and stomatal patterning. Phenotype, overview	Solanum lycopersicum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Solanum lycopersicum	5618	-
cell wall	-	Nicotiana benthamiana	5618	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	Solanum lycopersicum	-	CoA + 2-acyl-sn-glycerol 3-phosphate	-	?
acyl-CoA + sn-glycerol 3-phosphate	Nicotiana benthamiana	-	CoA + 2-acyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana benthamiana	-	-	-
Solanum lycopersicum	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
epidermis	-	Nicotiana benthamiana	-
flower	-	Solanum lycopersicum	-
flower	-	Nicotiana benthamiana	-
fruit	-	Solanum lycopersicum	-
fruit	-	Nicotiana benthamiana	-
leaf	-	Solanum lycopersicum	-
leaf	-	Nicotiana benthamiana	-
seed	-	Solanum lycopersicum	-
seed	-	Nicotiana benthamiana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	-	Solanum lycopersicum	CoA + 2-acyl-sn-glycerol 3-phosphate	-	?
acyl-CoA + sn-glycerol 3-phosphate	-	Nicotiana benthamiana	CoA + 2-acyl-sn-glycerol 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
glycerol-3-phosphate acyltransferase 6	-	Solanum lycopersicum
glycerol-3-phosphate acyltransferase 6	-	Nicotiana benthamiana
GPAT6	-	Solanum lycopersicum
GPAT6	-	Nicotiana benthamiana
NbGPAT6a	-	Nicotiana benthamiana
SlGPAT6a	-	Solanum lycopersicum

Expression

Organism	Comment	Expression
Solanum lycopersicum	gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830	up
Nicotiana benthamiana	gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830	up

General Information

General Information	Comment	Organism
malfunction	a loss-of-function mutation in tomato SlGPAT6 results in increased susceptibility of leaves to Phytophthora infection, concomitant with changes in haustoria morphology. Modulation of GPAT6 expression alters the outer wall diameter of leaf epidermal cells. Tomato gpat6-a mutants have an impaired cell wall-cuticle continuum and fewer stomata, but show increased water loss	Solanum lycopersicum
malfunction	silencing of Nicotiana benthamiana NbGPAT6a increases leaf susceptibility to infection by the oomycetes Phytophthora infestans and Phytophthora palmivora, whereas overexpression of NbGPAT6a-GFP renders the leaves more resistant. Knockdown or knockout of GPAT6 renders leaves more susceptible to Phytophthora infection but more resistant to Bortrytis cinerea infection	Nicotiana benthamiana
metabolism	cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position)	Solanum lycopersicum
metabolism	cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position)	Nicotiana benthamiana
additional information	constitutive expression of NbGPAT6a renders leaves resistant to Phytophthora infection, NbGPAT6-mediated resistance is associated with longer-term leaf development processes. Modulating GPAT6 expression alters the thickness of the outer cell walls of the leaf epidermis	Nicotiana benthamiana
physiological function	glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of Nicotiana benthamiana leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration	Nicotiana benthamiana
physiological function	glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration	Solanum lycopersicum

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Release 2023.1 (January 2023)