Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Listeria monocytogenes |
General Stability | Organism |
---|---|
loss of catalytic activity due to precipitation of purified Ptb is observed at concentrations ranging from 1-6 mg/ml in 50 mM Tris (pH 7.5) and in 25 mM HEPES (pH 7.5) buffers, which is resolved by storage in high NaCl concentrations (1.3-3 M) | Listeria monocytogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0315 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0315 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.0438 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0438 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.0481 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0481 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.0532 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0532 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.0579 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0579 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.0799 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.0799 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.1608 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.1608 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.1906 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
0.1906 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.992 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
0.9921 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
- |
- |
Listeria monocytogenes 10403S | - |
- |
- |
Storage Stability | Organism |
---|---|
can be stored for two weeks without detectable loss of activity by dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl | Listeria monocytogenes |
dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl, storage for two weeks without detectable loss of activity | Listeria monocytogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ethylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | 2-ethylbutanoyl phosphate + CoA | - |
? | |
2-ethylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + 2-ethylbutanoyl phosphate | - |
? | |
2-methylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | 2-methylbutanoyl phosphate + CoA | - |
? | |
2-methylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + 2-methylbutanoyl phosphate | - |
? | |
acetyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + acetyl phosphate | - |
? | |
acetyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | CoA + acetyl phosphate | - |
? | |
acetyl-CoA + phosphate | - |
Listeria monocytogenes | acetyl Phosphate + CoA | - |
? | |
acetyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | acetyl Phosphate + CoA | - |
? | |
butanoyl-CoA + phosphate | - |
Listeria monocytogenes | butanoyl phosphate + CoA | - |
? | |
butanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + butanoyl phosphate | - |
? | |
hexanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + hexanoyl phosphate | - |
? | |
hexanoyl-CoA + phosphate | - |
Listeria monocytogenes | hexanoyl phosphate + CoA | - |
? | |
isobutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + isobutanoyl phosphate | - |
? | |
isobutanoyl-CoA + phosphate | - |
Listeria monocytogenes | isobutanoyl phosphate + CoA | - |
? | |
isovaleryl-CoA + phosphate | - |
Listeria monocytogenes | CoA + isovaleryl phosphate | - |
? | |
isovaleryl-CoA + phosphate | - |
Listeria monocytogenes | isovaleryl phosphate + CoA | - |
? | |
additional information | enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation | Listeria monocytogenes | ? | - |
- |
|
additional information | mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain | Listeria monocytogenes | ? | - |
- |
|
additional information | enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation | Listeria monocytogenes 10403S | ? | - |
- |
|
additional information | mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain | Listeria monocytogenes 10403S | ? | - |
- |
|
pentanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + pentanoyl phosphate | - |
? | |
pentanoyl-CoA + phosphate | - |
Listeria monocytogenes | pentanoyl phosphate + CoA | - |
? | |
propionyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + propionyl phosphate | - |
? | |
propionyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | CoA + propionyl phosphate | - |
? | |
propionyl-CoA + phosphate | - |
Listeria monocytogenes | propionyl phosphate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Listeria monocytogenes |
Synonyms | Comment | Organism |
---|---|---|
PTB | - |
Listeria monocytogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
47 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
100.9 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
100.9 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
302.4 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
302.4 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
518.8 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
518.8 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
673.9 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
673.9 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
676.1 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
676.1 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
849.1 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
849.1 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
1043.4 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
1043.4 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
1263.4 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
1263.4 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
300 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
300 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
1700 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
1700 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2300 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2720 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2720 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
4200 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
4200 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
8430 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
8430 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
17700 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
17700 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
19600 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
19600 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
21400 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
21400 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
23000 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes |