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Literature summary for 2.3.1.19 extracted from

  • Sirobhushanam, S.; Galva, C.; Saunders, L.P.; Sen, S.; Jayaswal, R.; Wilkinson, B.J.; Gatto, C.
    Utilization of multiple substrates by butyrate kinase from Listeria monocytogenes (2017), Biochim. Biophys. Acta, 1862, 283-290 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Listeria monocytogenes

General Stability

General Stability Organism
loss of catalytic activity due to precipitation of purified Ptb is observed at concentrations ranging from 1-6 mg/ml in 50 mM Tris (pH 7.5) and in 25 mM HEPES (pH 7.5) buffers, which is resolved by storage in high NaCl concentrations (1.3-3 M) Listeria monocytogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0315
-
hexanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0315
-
hexanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.0438
-
2-ethylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0438
-
2-ethylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.0481
-
2-methylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0481
-
2-methylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.0532
-
isovaleryl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0532
-
isovaleryl-CoA pH 7.5, 23°C Listeria monocytogenes
0.0579
-
isobutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0579
-
isobutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.0799
-
butanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.0799
-
butanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.1608
-
pentanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.1608
-
pentanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.1906
-
propionyl-CoA pH 7.5, 25°C Listeria monocytogenes
0.1906
-
propionyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.992
-
acetyl-CoA pH 7.5, 23°C Listeria monocytogenes
0.9921
-
acetyl-CoA pH 7.5, 25°C Listeria monocytogenes

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
-
-
Listeria monocytogenes 10403S
-
-
-

Storage Stability

Storage Stability Organism
can be stored for two weeks without detectable loss of activity by dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl Listeria monocytogenes
dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl, storage for two weeks without detectable loss of activity Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ethylbutanoyl-CoA + phosphate
-
Listeria monocytogenes 2-ethylbutanoyl phosphate + CoA
-
?
2-ethylbutanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + 2-ethylbutanoyl phosphate
-
?
2-methylbutanoyl-CoA + phosphate
-
Listeria monocytogenes 2-methylbutanoyl phosphate + CoA
-
?
2-methylbutanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + 2-methylbutanoyl phosphate
-
?
acetyl-CoA + phosphate
-
Listeria monocytogenes CoA + acetyl phosphate
-
?
acetyl-CoA + phosphate
-
Listeria monocytogenes 10403S CoA + acetyl phosphate
-
?
acetyl-CoA + phosphate
-
Listeria monocytogenes acetyl Phosphate + CoA
-
?
acetyl-CoA + phosphate
-
Listeria monocytogenes 10403S acetyl Phosphate + CoA
-
?
butanoyl-CoA + phosphate
-
Listeria monocytogenes butanoyl phosphate + CoA
-
?
butanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + butanoyl phosphate
-
?
hexanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + hexanoyl phosphate
-
?
hexanoyl-CoA + phosphate
-
Listeria monocytogenes hexanoyl phosphate + CoA
-
?
isobutanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + isobutanoyl phosphate
-
?
isobutanoyl-CoA + phosphate
-
Listeria monocytogenes isobutanoyl phosphate + CoA
-
?
isovaleryl-CoA + phosphate
-
Listeria monocytogenes CoA + isovaleryl phosphate
-
?
isovaleryl-CoA + phosphate
-
Listeria monocytogenes isovaleryl phosphate + CoA
-
?
additional information enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation Listeria monocytogenes ?
-
-
additional information mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain Listeria monocytogenes ?
-
-
additional information enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation Listeria monocytogenes 10403S ?
-
-
additional information mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain Listeria monocytogenes 10403S ?
-
-
pentanoyl-CoA + phosphate
-
Listeria monocytogenes CoA + pentanoyl phosphate
-
?
pentanoyl-CoA + phosphate
-
Listeria monocytogenes pentanoyl phosphate + CoA
-
?
propionyl-CoA + phosphate
-
Listeria monocytogenes CoA + propionyl phosphate
-
?
propionyl-CoA + phosphate
-
Listeria monocytogenes 10403S CoA + propionyl phosphate
-
?
propionyl-CoA + phosphate
-
Listeria monocytogenes propionyl phosphate + CoA
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Listeria monocytogenes

Synonyms

Synonyms Comment Organism
PTB
-
Listeria monocytogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47
-
hexanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
47
-
hexanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
100.9
-
2-ethylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
100.9
-
2-ethylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
302.4
-
acetyl-CoA pH 7.5, 25°C Listeria monocytogenes
302.4
-
acetyl-CoA pH 7.5, 23°C Listeria monocytogenes
518.8
-
propionyl-CoA pH 7.5, 25°C Listeria monocytogenes
518.8
-
propionyl-CoA pH 7.5, 23°C Listeria monocytogenes
673.9
-
butanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
673.9
-
butanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
676.1
-
pentanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
676.1
-
pentanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
849.1
-
2-methylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
849.1
-
2-methylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
1043.4
-
isovaleryl-CoA pH 7.5, 25°C Listeria monocytogenes
1043.4
-
isovaleryl-CoA pH 7.5, 23°C Listeria monocytogenes
1263.4
-
isobutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
1263.4
-
isobutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
300
-
acetyl-CoA pH 7.5, 25°C Listeria monocytogenes
300
-
acetyl-CoA pH 7.5, 23°C Listeria monocytogenes
1700
-
hexanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
1700
-
hexanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
2300
-
2-ethylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
2720
-
propionyl-CoA pH 7.5, 25°C Listeria monocytogenes
2720
-
propionyl-CoA pH 7.5, 23°C Listeria monocytogenes
4200
-
pentanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
4200
-
pentanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
8430
-
butanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
8430
-
butanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
17700
-
2-methylbutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
17700
-
2-methylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
19600
-
isovaleryl-CoA pH 7.5, 25°C Listeria monocytogenes
19600
-
isovaleryl-CoA pH 7.5, 23°C Listeria monocytogenes
21400
-
isobutanoyl-CoA pH 7.5, 25°C Listeria monocytogenes
21400
-
isobutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes
23000
-
2-ethylbutanoyl-CoA pH 7.5, 23°C Listeria monocytogenes