Reference to 2.3.1.169; Id = 390475 - BRENDA Enzyme Database

CO

two effects: stimulation and inhibition on CoA/acetylCoA exchange

Moorella thermoacetica

reactions in presence of DTT, since enzyme requires strictly anaerobic conditions for stability

Moorella thermoacetica

CN-

inhibitor on the CoA/acetyl-CoA exchange, 98% inhibition at 1.2 mM

Moorella thermoacetica

CO

non-competitive inhibitor on the CoA/acetyl-CoA exchange, the Ni-Fe-C-center appears to be the inhibitor site for CO

Moorella thermoacetica

CO2

inhibitor on the CoA/acetyl-CoA exchange

Moorella thermoacetica

dephospho-CoA

inhibitor on the CoA/acetyl-CoA exchange, 75% inhibition at 0.44 mM

Moorella thermoacetica

desulfo-CoA

inhibitor on the CoA/acetyl-CoA exchange, 30% mM at 2.1 mM

Moorella thermoacetica

N2O

inhibitor on the CoA/acetyl-CoA exchange

Moorella thermoacetica

Fe

corrinoid/iron-sulfur protein required

Moorella thermoacetica

Fe

the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex

Moorella thermoacetica

Ni

the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex

Moorella thermoacetica

150000

-

Moorella thermoacetica

additional information

Moorella thermoacetica

key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway

?

-

?

Moorella thermoacetica

-

acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]

pathway

Moorella thermoacetica

acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]

Enzyme accepts the methyl group from the methylated corrinoid/iron-sulfur protein, binds a carbonyl group from CO, CO2, or the carboxyl of pyruvate, and binds coenzyme A. Then the enzyme catalyses the synthesis of acetyl-CoA from these enzyme bound groups. Additionally the enzyme catalyses two exchange reactions between the methylated corrinoid/iron-sulfur protein and methylated enzyme and between methylated enzyme and the methyl moiety of acetyl-CoA.

Moorella thermoacetica

acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]

the enzyme-bound complex is an [NiFe3-4S4]-acetyl complex

Moorella thermoacetica

28

-

40Â°C, CoA/acetyl-CoA exchange

Moorella thermoacetica

CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA

under anaerobic conditions

390475

Moorella thermoacetica

CH3-CO-S-CoA + corrinoid/iron-sulfur protein

-

390475

Moorella thermoacetica

?

CO + methyl-X + HS-CoA

-

390475

Moorella thermoacetica

CH3-CO-S-CoA + HX

-

390475

Moorella thermoacetica

?

additional information

enzyme catalyses the CoA/acetyl-CoA exchange

390475

Moorella thermoacetica

?

-

?

additional information

key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway

390475

Moorella thermoacetica

?

-

?

dimer

alpha,beta

Moorella thermoacetica

additional information

-

additional information

-

Moorella thermoacetica

6.7

7

optimum for CoA/acetyl-CoA exchange

Moorella thermoacetica

0.4

-

CO

pH 7.0, 25Â°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA

Moorella thermoacetica

CO

two effects: stimulation and inhibition on CoA/acetylCoA exchange

Moorella thermoacetica

reactions in presence of DTT, since enzyme requires strictly anaerobic conditions for stability

Moorella thermoacetica

CN-

inhibitor on the CoA/acetyl-CoA exchange, 98% inhibition at 1.2 mM

Moorella thermoacetica

CO

non-competitive inhibitor on the CoA/acetyl-CoA exchange, the Ni-Fe-C-center appears to be the inhibitor site for CO

Moorella thermoacetica

CO2

inhibitor on the CoA/acetyl-CoA exchange

Moorella thermoacetica

dephospho-CoA

inhibitor on the CoA/acetyl-CoA exchange, 75% inhibition at 0.44 mM

Moorella thermoacetica

desulfo-CoA

inhibitor on the CoA/acetyl-CoA exchange, 30% mM at 2.1 mM

Moorella thermoacetica

N2O

inhibitor on the CoA/acetyl-CoA exchange

Moorella thermoacetica

0.4

-

CO

pH 7.0, 25Â°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA

Moorella thermoacetica

Fe

corrinoid/iron-sulfur protein required

Moorella thermoacetica

Fe

the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex

Moorella thermoacetica

Ni

the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex

Moorella thermoacetica

150000

-

Moorella thermoacetica

additional information

Moorella thermoacetica

key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway

?

-

?

28

-

40Â°C, CoA/acetyl-CoA exchange

Moorella thermoacetica

CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA

under anaerobic conditions

390475

Moorella thermoacetica

CH3-CO-S-CoA + corrinoid/iron-sulfur protein

-

390475

Moorella thermoacetica

?

CO + methyl-X + HS-CoA

-

390475

Moorella thermoacetica

CH3-CO-S-CoA + HX

-

390475

Moorella thermoacetica

?

additional information

enzyme catalyses the CoA/acetyl-CoA exchange

390475

Moorella thermoacetica

?

-

?

additional information

key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway

390475

Moorella thermoacetica

?

-

?

dimer

alpha,beta

Moorella thermoacetica

additional information

-

additional information

-

Moorella thermoacetica

6.7

7

optimum for CoA/acetyl-CoA exchange

Moorella thermoacetica

758239

Adam

Evolutionary history of carbo ...

bacterium, archaeon

Proc. Natl. Acad. Sci. USA

115

E1166-E1173

2018

-

2

-

4

-

4

-

736020

Ali

Physiological characterization ...

Candidatus Jettenia caeni

Environ. Microbiol.

17

2172-2189

2015

-

2

-

1

-

3

-

2

-

1

-

728242

Zhu

Structural and functional inve ...

Clostridioides difficile, Clostridioides difficile 630

Metallomics

5

551-558

2013

-

1

-

2

-

2

-

8

-

2

-

1

-

1

-

2

-

2

-

2

-

1

736683

Chmielowska

Redox potentials and protonati ...

Carboxydothermus hydrogenoformans

J. Phys. Chem. A

117

12484-12496

2013

-

1

-

1

-

1

-

1

-

1

-

719744

Matschiavelli

Function and regulation of iso ...

Methanosarcina acetivorans

J. Bacteriol.

194

5377-5387

2012

-

1

-

1

-

5

-

1

-

6

-

1

-

1

-

1

-

1

-

1

-

2

-

714240

Bender

Evidence that ferredoxin inter ...

Moorella thermoacetica

Biochemistry

50

276-286

2011

1

-

1

-

3

-

1

-

1

-

1

-

1

-

2

1

-

2

-

2

-

2

-

1

-

1

2

-

3

-

1

-

1

-

1

-

2

1

2

-

2

-

1

-

720372

Grahame

Methods for analysis of acetyl ...

Carboxydothermus hydrogenoformans

Methods Enzymol.

494

189-217

2011

-

1

-

3

-

1

-

1

-

2

-

2

1

-

2

-

1

-

3

-

1

-

2

-

1

-

2

-

1

-

719862

Gencic

Tight coupling of partial reac ...

Carboxydothermus hydrogenoformans, Methanosarcina thermophila, Carboxydothermus hydrogenoformans DSM 6008, Methanosarcina thermophila TM-1

J. Biol. Chem.

285

15450-15463

2010

-

2

-

7

-

3

-

4

-

10

-

2

-

8

1

4

2

-

3

-

2

-

7

-

3

-

4

-

2

-

8

1

2

-

3

-

6

-

696351

Kung

Crystallographic snapshots of ...

Moorella thermoacetica

Biochemistry

48

7432-7440

2009

-

1

-

1

-

1

-

696354

Volbeda

Novel domain arrangement in th ...

Moorella thermoacetica

Biochemistry

48

7916-7926

2009

-

1

2

-

2

-

1

-

2

-

700996

Ito

Dinuclear nickel complexes mod ...

synthetic construct

Proc. Natl. Acad. Sci. USA

106

11862-11866

2009

-

1

-

1

-

1

-

685228

Doukov

Xenon in and at the end of the ...

Moorella thermoacetica

Biochemistry

47

3474-3483

2008

-

1

-

1

-

1

-

687803

Seravalli

Pulse-chase studies of the syn ...

Moorella thermoacetica

J. Biol. Chem.

283

8384-8394

2008

-

1

-

1

-

1

-

1

-

1

-

1

-

687824

Tan

Tunnel mutagenesis and Ni-depe ...

Moorella thermoacetica

J. Biol. Inorg. Chem.

13

771-778

2008

-

2

-

2

-

1

-

1

-

2

-

2

-

672199

Bramlett

Moessbauer and EPR study of re ...

Moorella thermoacetica

Biochemistry

45

8674-8685

2006

-

2

-

1

-

1

-

2

-

674177

Tan

Kinetics of CO insertion and a ...

Moorella thermoacetica

J. Am. Chem. Soc.

128

12331-12338

2006

-

1

-

1

-

1

-

1

-

674925

Tan

Function of the tunnel in acet ...

Moorella thermoacetica

J. Biol. Inorg. Chem.

11

371-378

2006

-

5

-

1

-

5

-

655801

Tan

The tunnel of acetyl-coenzyme ...

Moorella thermoacetica

J. Am. Chem. Soc.

127

5833-5839

2005

-

3

-

1

-

1

-

2

-

1

-

3

-

1

-

674161

Amara

A quantum chemical study of th ...

synthetic construct

J. Am. Chem. Soc.

127

2776-2784

2005

-

1

-

1

-

660370

Svetlitchnyi

A functional Ni-Ni-[4Fe-4S] cl ...

Carboxydothermus hydrogenoformans

Proc. Natl. Acad. Sci. USA

101

446-451

2004

-

1

3

2

-

1

-

1

-

1

-

1

3

2

-

1

-

1

-

644862

Tan

Reduction and methyl transfer ...

Moorella thermoacetica

J. Am. Chem. Soc.

125

318-319

2003

-

4

-

1

-

2

-

1

-

4

-

1

-

644863

Bramlett

Inactivation of acetyl-CoA syn ...

Moorella thermoacetica

J. Am. Chem. Soc.

125

9316-9317

2003

-

3

-

1

-

1

-

3

-

1

-

658436

Golden

-

Capture of NiII, CuI and ZnII ...

Moorella thermoacetica

Chem. Commun. (Camb.)

2003

1824-1825

2003

-

3

-

1

-

1

-

3

-

658973

Linck

Structural analogues of the bi ...

Moorella thermoacetica

J. Am. Chem. Soc.

125

8700-8701

2003

-

1

-

1

-

1

-

660349

Seravalli

Functional copper at the acety ...

Moorella thermoacetica

Proc. Natl. Acad. Sci. USA

100

3689-3694

2003

-

3

-

1

-

3

-

644679

Doukov

A Ni-Fe-Cu center in a bifunct ...

Moorella thermoacetica

Science

298

567-572

2002

-

1

-

3

1

-

1

-

1

2

3

-

1

-

3

1

-

1

2

-

2

-

644861

Tan

Stopped-flow kinetics of methy ...

Moorella thermoacetica

J. Am. Chem. Soc.

124

6277-6284

2002

-

3

-

2

-

1

-

1

-

2

-

2

-

3

-

2

-

1

-

2

-

644864

Seravalli

Acetyl Coenzyme A synthesis fr ...

Moorella thermoacetica, Methanosarcina barkeri

J. Am. Chem. Soc.

123

1786-1787

2001

-

2

-

2

-

2

-

2

-

8

-

2

-

2

-

8

-

644858

Murakami

Evidence for intersubunit comm ...

Methanosarcina thermophila, Methanosarcina thermophila TM1

J. Biol. Chem.

275

4699-4707

2000

-

3

-

5

-

6

1

-

3

-

6

1

-

644857

Menon

The role of an iron-sulfur clu ...

Moorella thermoacetica

J. Biol. Chem.

274

11513-11518

1999

-

2

-

1

-

1

-

2

-

3

-

2

-

1

-

3

-

644678

Menon

Role of the [4Fe-4S] cluster i ...

Moorella thermoacetica

Biochemistry

37

5689-5698

1998

-

2

-

1

-

1

-

2

-

4

-

2

-

1

-

4

-

644855

Kasmi

Anaerobic pathway for conversi ...

Moorella thermoacetica

Biochemistry

33

11217-11224

1994

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

3

-

390465

Grahame

Substrate and cofactor reactiv ...

Methanosarcina barkeri

Biochemistry

32

10786-10793

1993

-

1

-

1

-

2

-

4

-

1

-

4

-

644854

Jablonski

Characterization of the metal ...

Methanosarcina thermophila

J. Biol. Chem.

268

325-329

1993

-

4

-

4

-

1

-

1

-

4

-

1

-

644674

Roberts

Acetyl-coenzyme A synthesis fr ...

Moorella thermoacetica

J. Bacteriol.

174

4667-4676

1992

1

-

1

-

1

-

1

-

1

-

1

2

-

5

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

5

-

5

-

1

-

1

-

390474

Grahame

Catalysis of acetyl-CoA cleava ...

Methanosarcina barkeri

J. Biol. Chem.

266

22227-22233

1991

-

1

6

-

1

-

1

-

1

-

1

6

-

1

-

1

-

390475

Lu

Reductive activation of the co ...

Moorella thermoacetica

J. Biol. Chem.

266

3554-3564

1991

1

-

1

6

-

3

1

-

1

-

3

-

1

-

4

1

-

1

-

1

-

1

-

1

-

6

1

-

3

1

-

1

-

4

1

-

1

-

644850

Lu

Controlled potential enzymolog ...

Moorella thermoacetica

J. Biol. Chem.

265

3124-3133

1990

1

-

1

2

-

2

-

1

-

1

-

1

2

-

4

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

4

-

1

-

644851

Roberts

Cloning and expression of the ...

Moorella thermoacetica

Proc. Natl. Acad. Sci. USA

86

32-36

1989

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

2

-

390459

Ragsdale

Acetate biosynthesis by acetog ...

Moorella thermoacetica

J. Biol. Chem.

260

3970-3977

1985

1

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

Reductive activation of the coenzyme A/acetyl-CoA isotopic exchange reaction catalyzed by carbon monoxide dehydrogenase from Clostridium thermoaceticum and its inhibition by nitrous oxide and carbon monoxide

Data extracted from this reference: