Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CO | two effects: stimulation and inhibition on CoA/acetylCoA exchange | Moorella thermoacetica |
General Stability | Organism |
---|---|
reactions in presence of DTT, since enzyme requires strictly anaerobic conditions for stability | Moorella thermoacetica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | inhibitor on the CoA/acetyl-CoA exchange, 98% inhibition at 1.2 mM | Moorella thermoacetica | |
CO | non-competitive inhibitor on the CoA/acetyl-CoA exchange, the Ni-Fe-C-center appears to be the inhibitor site for CO | Moorella thermoacetica | |
CO2 | inhibitor on the CoA/acetyl-CoA exchange | Moorella thermoacetica | |
dephospho-CoA | inhibitor on the CoA/acetyl-CoA exchange, 75% inhibition at 0.44 mM | Moorella thermoacetica | |
desulfo-CoA | inhibitor on the CoA/acetyl-CoA exchange, 30% mM at 2.1 mM | Moorella thermoacetica | |
N2O | inhibitor on the CoA/acetyl-CoA exchange | Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | corrinoid/iron-sulfur protein required | Moorella thermoacetica | |
Fe | the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex | Moorella thermoacetica | |
Ni | the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
- |
Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Moorella thermoacetica | key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | pathway | Moorella thermoacetica | |
acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | Enzyme accepts the methyl group from the methylated corrinoid/iron-sulfur protein, binds a carbonyl group from CO, CO2, or the carboxyl of pyruvate, and binds coenzyme A. Then the enzyme catalyses the synthesis of acetyl-CoA from these enzyme bound groups. Additionally the enzyme catalyses two exchange reactions between the methylated corrinoid/iron-sulfur protein and methylated enzyme and between methylated enzyme and the methyl moiety of acetyl-CoA. | Moorella thermoacetica | |
acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | the enzyme-bound complex is an [NiFe3-4S4]-acetyl complex | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
40°C, CoA/acetyl-CoA exchange | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA | under anaerobic conditions | Moorella thermoacetica | CH3-CO-S-CoA + corrinoid/iron-sulfur protein | - |
? | |
CO + methyl-X + HS-CoA | - |
Moorella thermoacetica | CH3-CO-S-CoA + HX | - |
? | |
additional information | enzyme catalyses the CoA/acetyl-CoA exchange | Moorella thermoacetica | ? | - |
? | |
additional information | key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway | Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha,beta | Moorella thermoacetica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7 | optimum for CoA/acetyl-CoA exchange | Moorella thermoacetica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
CO | pH 7.0, 25°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA | Moorella thermoacetica |