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Literature summary for 2.3.1.168 extracted from
- Avermectin biosynthesis stable functional expression of branched chain alpha-keto acid dehydrogenase complex from Streptomyces avermitilis in Escherichia coli by selectively regulating individual subunit gene expression (2017), Biotechnol. Lett., 39, 1567-1574 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | functional expression of the branched chain alpha-keto acid dehydrogenase complex in Escherichia coli by independent and selective optimization of individual subunit genes of the complex. Codon optimization significantly improves the expression of complex component proteins BkdH and LpdA1 but expression of dehydrogenase E1 alpha subunit BkdF and dehydrogenase E1 beta subunit BkdG depends on coexpression of the BkdH gene. The optimized branched chain alpha-keto acid dehydrogenase complex supplies sufficient short branched-chain acyl-CoA to synthesize phlorisovalerophenone | Streptomyces avermitilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces avermitilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BKDH | - |
Streptomyces avermitilis |
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Release 2023.1 (January 2023)
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