BRENDA - Enzyme Database
show all sequences of 2.3.1.158

Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis

Stahl, U.; Carlsson, A.S.; Lenman, M.; Dahlqvist, A.; Huang, B.; Banas, W.; Banas, A.; Stymne, S.; Plant Physiol. 135, 1324-1335 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA sequence determination and analysis, functional overexpression in Arabidopsis thaliana roots and leaves
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic plants overexpressing the endogenous gene in leaves and roots, the transgenic plants show elevated enzyme activity
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phospholipid + 1,2-diacylglycerol
Arabidopsis thaliana
-
lysophospholipid + triacylglycerol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9FNA9
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
enzyme contains 4 potential N-glycosylation sites at residues N161, N381, N434, and N647
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phospholipid + 1,2-diacylglycerol
-
660241
Arabidopsis thaliana
lysophospholipid + triacylglycerol
-
-
-
?
phospholipid + 1,2-diacylglycerol
enzyme accepts acyl groups ranging from C10 to C22, activity is highly dependent on the acyl composition preferring acyl groups with several double bonds, epoxy, or hydroxy groups, enzyme has a 3fold preference for the sn-2 compared to sn-1 position of phosphatidylcholine, overview
660241
Arabidopsis thaliana
lysophospholipid + triacylglycerol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA sequence determination and analysis, functional overexpression in Arabidopsis thaliana roots and leaves
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic plants overexpressing the endogenous gene in leaves and roots, the transgenic plants show elevated enzyme activity
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phospholipid + 1,2-diacylglycerol
Arabidopsis thaliana
-
lysophospholipid + triacylglycerol
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
enzyme contains 4 potential N-glycosylation sites at residues N161, N381, N434, and N647
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phospholipid + 1,2-diacylglycerol
-
660241
Arabidopsis thaliana
lysophospholipid + triacylglycerol
-
-
-
?
phospholipid + 1,2-diacylglycerol
enzyme accepts acyl groups ranging from C10 to C22, activity is highly dependent on the acyl composition preferring acyl groups with several double bonds, epoxy, or hydroxy groups, enzyme has a 3fold preference for the sn-2 compared to sn-1 position of phosphatidylcholine, overview
660241
Arabidopsis thaliana
lysophospholipid + triacylglycerol
-
-
-
?
Other publictions for EC 2.3.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Functional study of diacylglyc ...
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FEBS Lett.
587
2364-2370
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1
3
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736422
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Linum usitatissimum
J. Biol. Chem.
288
24173-24188
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1
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2
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2
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1
2
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736985
Fan
Dual role for phospholipid:dia ...
Arabidopsis thaliana
Plant Cell
25
3506-3518
2013
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1
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1
1
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720694
Yoon
Phospholipid:diacylglycerol ac ...
Chlamydomonas reinhardtii
Plant Cell
24
3708-3724
2012
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2
1
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2
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3
1
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2
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1
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1
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2
1
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1
1
3
3
1
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721119
Zhang
Three diacylglycerol acyltrans ...
Yarrowia lipolytica, Yarrowia lipolytica ATCC 20362
Yeast
29
25-38
2012
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1
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1
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5
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1
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720737
van Erp
Castor phospholipid:diacylglyc ...
Ricinus communis
Plant Physiol.
155
683-693
2011
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1
1
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1
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1
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697014
Dauk
-
The role of diacylglycerol acy ...
Arabidopsis thaliana
Botany
87
552-560
2009
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1
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1
1
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684576
Arabolaza
Multiple pathways for triacylg ...
Streptomyces coelicolor
Appl. Environ. Microbiol.
74
2573-2582
2008
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4
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687725
Czabany
Structural and biochemical pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
17065-17074
2008
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688089
Hernandez
The utilization and desaturati ...
Olea europaea
J. Exp. Bot.
59
2425-2435
2008
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685341
Ghosal
Saccharomyces cerevisiae phosp ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1771
1457-1463
2007
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1
1
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676641
Mhaske
Isolation and characterization ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
413-417
2005
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660241
Stahl
Cloning and functional charact ...
Arabidopsis thaliana
Plant Physiol.
135
1324-1335
2004
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1
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1
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1
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1
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6
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1
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2
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2
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1
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1
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1
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1
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1
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2
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2
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393081
Banas
The involvement of phospholipi ...
Arabidopsis thaliana, Crepis palaestina, Euphorbia lagascae, Ricinus communis
Biochem. Soc. Trans.
28
703-705
2000
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9
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18
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4
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18
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393082
Dahlqvist
Phospholipid:diacylglycerol ac ...
Crepis palaestina, Helianthus annuus, Ricinus communis, Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
97
6487-6492
2000
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1
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4
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10
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3
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24
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1
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24
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