Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipids | - |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Monoacylglycerophosphate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Escherichia coli | 5743 | - |
plasma membrane | - |
Escherichia coli | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
solubilized | Escherichia coli |
solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 1-acyl-sn-glycerol 3-phosphate | mechanism | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution in phosphatidylcholine/phosphatidylglycerol, ratio 4:1, vesicles, 90% of active site of the reconstituted enzyme are located at the outside surface | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | temperature-dependent specificity of reconstituted enzyme | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | saturated acyl-CoAs preferred | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
reconstituted enzyme | Escherichia coli |