BRENDA - Enzyme Database show
show all sequences of 2.2.1.9

MenD as a versatile catalyst for asymmetric synthesis

Kurutsch, A.; Richter, M.; Brecht, V.; Sprenger, G.; Müller, M.; J. Mol. Catal. B 61, 56-66 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21
Escherichia coli K-12
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli K-12
-
-
-
Purification (Commentary)
Commentary
Organism
gel permeation chromatography
Escherichia coli K-12
Storage Stability
Storage Stability
Organism
4°C, desalting buffer with 50 mM phosphate, 1 mM thiamine diphosphate and 2 mM MgCl2, pH 8.0, 4 weeks, no significant loss of activity
Escherichia coli K-12
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2,3-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-bromobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-bromophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-chlorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-fluorobenzaldehyde + oxaloacetate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + 2 CO2
-
-
-
-
2-fluorobenzaldehyde + pyruvate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + CO2
-
-
-
-
2-iodobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(2-iodophenyl)-4-oxopentanoic acid + CO2
-
-
-
-
2-methylbenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(2-methylphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
3,4-dihydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
3-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(3-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
3-iodobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(3-iodophenyl)-4-oxopentanoic acid + CO2
-
-
-
-
3-methoxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(3-methoxyphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
4-chlorbenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(4-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
4-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(4-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
4-hydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(4-hydroxyphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
acetaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxohexanoic acid + CO2
-
-
-
-
benzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxo-5-phenylpentanoic acid + CO2
-
-
-
-
benzaldehyde + oxaloacetate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
-
-
-
benzaldehyde + pyruvate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
-
-
-
cyclohex-1-ene-1-carbaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-cyclohex-1-en-1-yl-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
glyoxylate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
5-hydroxy-4-oxopentanoic acid + 2 CO2
-
-
-
-
hexanal + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxodecanoic acid + CO2
-
-
-
-
isochorismate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(1R,2S,5S,6S)-5-[(1-carboxyethenyl)oxy]-2-(3-carboxypropanoyl)-6-hydroxycyclohex-3-ene-1-carboxylic acid + CO2
-
-
-
-
pyruvate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
4-hydroxy-5-oxohexanoic acid + 2 CO2
-
-
-
-
undec-10-enal + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxopentadec-14-enoic acid + CO2
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli K-12
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Escherichia coli K-12
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21
Escherichia coli K-12
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Escherichia coli K-12
Purification (Commentary) (protein specific)
Commentary
Organism
gel permeation chromatography
Escherichia coli K-12
Storage Stability (protein specific)
Storage Stability
Organism
4°C, desalting buffer with 50 mM phosphate, 1 mM thiamine diphosphate and 2 mM MgCl2, pH 8.0, 4 weeks, no significant loss of activity
Escherichia coli K-12
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2,3-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-bromobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-bromophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-chlorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(2-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
2-fluorobenzaldehyde + oxaloacetate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + 2 CO2
-
-
-
-
2-fluorobenzaldehyde + pyruvate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + CO2
-
-
-
-
2-iodobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(2-iodophenyl)-4-oxopentanoic acid + CO2
-
-
-
-
2-methylbenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(2-methylphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
3,4-dihydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
3-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(3-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
3-iodobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(3-iodophenyl)-4-oxopentanoic acid + CO2
-
-
-
-
3-methoxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(3-methoxyphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
4-chlorbenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(4-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
4-fluorobenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-(4-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
4-hydroxybenzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-5-(4-hydroxyphenyl)-4-oxopentanoic acid + CO2
-
-
-
-
acetaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxohexanoic acid + CO2
-
-
-
-
benzaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxo-5-phenylpentanoic acid + CO2
-
-
-
-
benzaldehyde + oxaloacetate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
-
-
-
benzaldehyde + pyruvate
assay at pH 8
705201
Escherichia coli K-12
(1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
-
-
-
cyclohex-1-ene-1-carbaldehyde + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-cyclohex-1-en-1-yl-5-hydroxy-4-oxopentanoic acid + CO2
-
-
-
-
glyoxylate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
5-hydroxy-4-oxopentanoic acid + 2 CO2
-
-
-
-
hexanal + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxodecanoic acid + CO2
-
-
-
-
isochorismate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(1R,2S,5S,6S)-5-[(1-carboxyethenyl)oxy]-2-(3-carboxypropanoyl)-6-hydroxycyclohex-3-ene-1-carboxylic acid + CO2
-
-
-
-
pyruvate + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
4-hydroxy-5-oxohexanoic acid + 2 CO2
-
-
-
-
undec-10-enal + 2-oxoglutarate
assay at pH 8
705201
Escherichia coli K-12
(5R)-5-hydroxy-4-oxopentadec-14-enoic acid + CO2
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli K-12
Other publictions for EC 2.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745170
Song
A thiamine-dependent enzyme u ...
Escherichia coli
J. Am. Chem. Soc.
138
7244-7247
2016
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1
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1
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1
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2
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1
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2
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1
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3
3
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718915
Fang
Using substrate analogues to p ...
Escherichia coli, Mycobacterium tuberculosis
Biochemistry
50
8712-8721
2011
-
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12
-
4
22
-
2
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2
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1
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18
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22
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18
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1
1
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15
15
720243
Dawson
Structure and reactivity of Ba ...
Bacillus subtilis
J. Mol. Biol.
401
253-264
2010
-
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1
8
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7
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1
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2
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1
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1
1
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9
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8
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1
1
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9
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9
9
702035
Priyadarshi
Structural insights of the Men ...
Escherichia coli K-12
Biochem. Biophys. Res. Commun.
380
797-801
2009
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1
1
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3
-
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1
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1
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705201
Kurutsch
-
MenD as a versatile catalyst f ...
Escherichia coli K-12
J. Mol. Catal. B
61
56-66
2009
-
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1
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1
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25
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1
25
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1
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705130
Dawson
Specificity and reactivity in ...
Escherichia coli K-12
J. Mol. Biol.
384
1353-1368
2008
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685086
Jiang
Menaquinone biosynthesis in Es ...
Escherichia coli
Biochemistry
46
10979-10989
2007
1
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684159
Sieminska
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. F
61
489-492
2005
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