Literature summary for 2.2.1.1 extracted from

Saravanan, T.; Reif, M.; Yi, D.; Lorilliere, M.; Charmantray, F.; Hecquet, L.; Fessner, W.
Engineering a thermostable transketolase for arylated substrates (2017), Green Chem., 19, 481-489 .

No PubMed abstract available

Search for more literature for 2.2.1.1

Protein Variants

Protein Variants	Comment	Organism
D470T	the mutant shows slightly increased activity with benzaldehyde compared to the wild type enzyme	Geobacillus stearothermophilus
L382D/D470S	the mutant shows strongly increased activity with benzaldehyde compared to the wild type enzyme	Geobacillus stearothermophilus
L382E/D470T	the mutant shows increased activity with benzaldehyde compared to the wild type enzyme	Geobacillus stearothermophilus
L382N/D470S	the mutant shows strongly increased activity with benzaldehyde compared to the wild type enzyme	Geobacillus stearothermophilus
L382N/D470T	the mutant shows increased activity with benzaldehyde compared to the wild type enzyme	Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
fructose 6-phosphate + D-glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phenylethanal + hydroxypyruvate	-	Geobacillus stearothermophilus	(S)-1,3-dihydroxy-4-phenylbutan-2-one + CO2	-	ir
3-phenylpropanal + hydroxypyruvate	-	Geobacillus stearothermophilus	(S)-1,3-dihydroxy-5-phenylpentan-2-one + CO2	-	ir
benzaldehyde + hydroxypyruvate	the wild type enzyme shows very little activity against benzaldehyde	Geobacillus stearothermophilus	(R)-1,3-dihydroxy-1-phenylpropan-2-one + CO2	-	ir
fructose 6-phosphate + D-glyceraldehyde 3-phosphate	-	Geobacillus stearothermophilus	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Geobacillus stearothermophilus	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)