Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F434A | the mutation leads to 53% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal) | Escherichia coli |
F434L | the mutation leads to 74% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H261A | the mutation leads to 75% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H261F | the mutation leads to 33% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H261G | the mutation leads to 59% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H261L | the mutation leads to 55% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H261V | the mutation leads to 65% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
H26A | the mutation results in stereoinversion for the formation of 1,3-dihydroxy-2-heptanone, with a lower ee-value of 18% (R) as compared to the wild type enzyme | Escherichia coli |
H26A/H261A | the mutation leads to 54% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal) | Escherichia coli |
H26W | the mutant shows an 8fold decreased formation of (R)-1,3-dihydroxy-2-heptanone and an ee-value of 30% (S) after 1 h reaction time | Escherichia coli |
H26Y | the mutant catalyzes the formation of (R)-1,3-dihydroxy-2-heptanone with an ee-value of 98% and a yield of 8% after 1 h | Escherichia coli |
L382A | the mutation leads to 97% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
L382A/F434A | the mutation leads to 66% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
L382A/F434L | the mutation leads to 10% ee (S) after 1 h in the reaction of 3-hydroxypyruvate and n-pentanal | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | Escherichia coli | - |
D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27302 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypyruvate + n-pentanal | 3% yield after 1 h (92% (S)), 12% yield after 3 h (87% (S)) | Escherichia coli | 1,3-dihydroxy-2-heptanone + CO2 | - |
ir | |
3-hydroxypyruvate + propanal | 12% yield after 1 h (57% (S)), 32% yield after 3 h (53% (S)) | Escherichia coli | 1,3-dihydroxy-2-pentanone + CO2 | - |
ir | |
D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
Escherichia coli | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TktA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Escherichia coli |