Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells | Staphylococcus aureus |
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 1 M NaH2PO4, 1 M K2HPO4, 0.2 M Li2SO4, and 0.1 M CAPS at pH 9.5 | Escherichia coli |
vapor diffusion method, using 30% (w/v) PEG-400, 0.1 M HEPES at pH 8.0, and 0.2 M MgCl2 | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA | Staphylococcus aureus | - |
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA | - |
? | |
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA | Escherichia coli | - |
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABD5 | - |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
Ni-NTA resin column chromatography, Hi-Trap column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA | - |
Staphylococcus aureus | [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA | - |
? | |
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA | - |
Escherichia coli | [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA carboxylase carboxyltransferase domain | - |
Staphylococcus aureus |
acetyl-CoA carboxylase carboxyltransferase domain | - |
Escherichia coli |