Cloned (Comment) | Organism |
---|---|
5S subunit of enzyme | Propionibacterium freudenreichii subsp. shermanii |
the gene encoding the 5S subunit is cloned into the pTXB1 vector | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55500 | - |
x * 55620, calculated, x * 55623, ESI-MS, x * 55500, SDS-PAGE of 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
55620 | - |
x * 55620, calculated, x * 55623, ESI-MS, x * 55500, SDS-PAGE of 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
55623 | - |
x * 55620, calculated, x * 55623, ESI-MS, x * 55500, SDS-PAGE of 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
55700 | - |
5S subunit, 505 amino acids | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate | Propionibacterium freudenreichii subsp. shermanii | - |
propanoyl-CoA + oxaloacetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Propionibacterium freudenreichii subsp. shermanii | - |
Propionibacterium f. shermanii | - |
Purification (Comment) | Organism |
---|---|
recombinant 5S subunit, single step purification using Intein mediated protein ligation method and cleavage from beads by dithiothreitol | Propionibacterium freudenreichii subsp. shermanii |
the expressed 5S subunit is purified to apparent homogeneity by a single step process by using Intein mediated protein ligation method | Propionibacterium freudenreichii subsp. shermanii |
Storage Stability | Organism |
---|---|
-70°C, recombinant 5S subunit purified using Intein mediated protein ligation method and cleavage from beads by dithiothreitol, stable for months | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate | - |
Propionibacterium freudenreichii subsp. shermanii | propanoyl-CoA + oxaloacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 55620, calculated, x * 55623, ESI-MS, x * 55500, SDS-PAGE of 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
multimer | the central cylindrical hexameric 12S subunit, the outer six dimeric 5S subunit, and the twelve 1.3S linkers | Propionibacterium freudenreichii subsp. shermanii |
Synonyms | Comment | Organism |
---|---|---|
5S subunit of transcarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii |
methylmalonyl CoA-oxalacetate transcarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii |
transcarboxylase | - |
Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Propionibacterium freudenreichii subsp. shermanii |