Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
N-formyltransferase domain of the enzyme in complex with N5-formyltetrahydrofolate and UDP-4-amino-4-deoxy-beta-L-arabinopyranose, hanging drop vapor diffusion method, using 20-22% poly(ethyleneglycol) 5000, 50 mM MgCl2, 100 mM HEPES (pH 8.0) at 21°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | Escherichia coli | - |
5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? | |
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | Escherichia coli W3110 | - |
5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | F4SGI5 | - |
- |
Escherichia coli W3110 | F4SGI5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? | |
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | - |
Escherichia coli W3110 | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? | |
additional information | the bifunctional enzyme has N- and C-terminal domains catalyzing formylation and oxidative decarboxylation reactions, respectively | Escherichia coli | ? | - |
- |
|
additional information | the bifunctional enzyme has N- and C-terminal domains catalyzing formylation and oxidative decarboxylation reactions, respectively | Escherichia coli W3110 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
hexamer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ArnA | - |
Escherichia coli |
Pmrl | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the formation of 4-amino-4-deoxy-L-arabinose. The addition of this sugar to the lipid A moiety of the lipopolysaccharide of pathogenic Gram-negative bacteria allows these organisms to evade the cationic antimicrobial peptides of the host immune system | Escherichia coli |