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Literature summary for 2.1.1.B141 extracted from

  • Ellenbogen, J.; Jiang, R.; Kountz, D.; Zhang, L.; Krzycki, J.
    The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent gamma-butyrobetaine methyltransferase (2021), J. Biol. Chem., 297, 101327 .
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
 gamma-butyrobetaine pH 7.2, 37°C Eubacterium limosum
4
-
 L-carnitine pH 7.2, 37°C Eubacterium limosum

Organism

Organism UniProt Comment Textmining
Eubacterium limosum A0A317RJG9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + 2-(dimethylamino)ethan-1-ol
-
 ?
gamma-butyrobetaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + 4-(dimethylamino)butanoate
-
 ?
glycine betaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + N-dimethylglycine
-
 ?
L-carnitine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + L-norcarnitine
-
 ?
additional information demethylation of other methylated amines occurs at much lower rates than observed with gamma-butyrobetaine or L-carnitine as a substrate Eubacterium limosum ?
-
-
proline betaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + N-methylproline
-
 ?

Synonyms

Synonyms Comment Organism
C7955_113110
-
 Eubacterium limosum
MtyB
-
 Eubacterium limosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 gamma-butyrobetaine pH 7.2, 37°C Eubacterium limosum
6.8
-
 L-carnitine pH 7.2, 37°C Eubacterium limosum

Expression

Organism Comment Expression
Eubacterium limosum MtyB constitutes 0.9 mol% from gamma-butyrobetaine-grown cells but is not detectable in the proteome of cells grown on lactate additional information

General Information

General Information Comment Organism
physiological function proteins MtyB, MtqC (a corrinoid-binding protein), and MtqA (a corrinoid:tetrahydrofolate methyltransferase) are significantly elevated in gamma-butyrobetaine-grown cells. Together, they act as a gamma-butyrobetaine:tetrahydrofolate methyltransferase system, forming a key intermediate of acetogenesis. Recombinant MtyB acts as a gamma-butyrobetaine:MtqC methyltransferase but cannot methylate free cobalamin cofactor Eubacterium limosum