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Literature summary for 2.1.1.B121 extracted from

  • Li, J.; Li, C.; Gou, J.; Zhang, Y.
    Molecular cloning and functional characterization of a novel isoflavone 3'-O-methyltransferase from Pueraria lobata (2016), Front. Plant Sci., 7, 793 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Pueraria montana var. lobata

Organism

Organism UniProt Comment Textmining
Pueraria montana var. lobata A0A1L1Z440
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf very poor expression Pueraria montana var. lobata
-
root highest expression level Pueraria montana var. lobata
-
stem very poor expression Pueraria montana var. lobata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3'-hydroxydaidzein
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + 3'-methoxydaidzein
-
 ?
S-adenosyl-L-methionine + 3'-hydroxypuerarin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + 3'-methoxypuerarin
-
 ?
S-adenosyl-L-methionine + luteolin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + chrysoeriol
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + isorhamnetin
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE, recombinant protein Pueraria montana var. lobata

Synonyms

Synonyms Comment Organism
OMT4
-
 Pueraria montana var. lobata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pueraria montana var. lobata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pueraria montana var. lobata

Expression

Organism Comment Expression
Pueraria montana var. lobata application of methyl jasmonate significantly increases transcript levels of OMT4 and biosynthesis of 3'-methoxydaidzein up