Literature summary for 2.1.1.9 extracted from

  • Borchardt, R.T.; Cheng, C.F.
    Purification and characterization of rat liver microsomal thiol methyltransferase (1978), Biochim. Biophys. Acta, 522, 340-353.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information overview: inhibition by analogs of S-adenosyl-L-homocysteine and analogs of S-adenosyl-L-methionine Rattus norvegicus
N-ethylmaleimide more than 50% inhibition at 0.2 mM Rattus norvegicus
p-chloromercuribenzoate more than 50% inhibition at 0.1 mM Rattus norvegicus
S-3-deazaadenosyl-L-homocysteine
-
Rattus norvegicus
S-adenosyl-L-homocysteine
-
Rattus norvegicus
S-Tubercidinyl-L-homocysteine
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
S-Aristeromycinyl-L-methionine
-
Rattus norvegicus
0.12
-
S-3'-Deoxyadenosyl-L-methionine
-
Rattus norvegicus
0.154
-
S-Tubercidinyl-L-methionine
-
Rattus norvegicus
0.106
-
S-adenosyl-L-methionine
-
Rattus norvegicus
0.224
-
S-3-Deazaadenosyl-L-methionine
-
Rattus norvegicus
2.37
-
2-mercaptoethanol
-
Rattus norvegicus
0.719
-
S-8-Azaadenosyl-L-methionine
-
Rattus norvegicus
1.017
-
S-N6-Methyladenosyl-L-methionine
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no stimulation by: Na+, K+, Mn2+ Rattus norvegicus
additional information no stimulation by: Mg2+ Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, 140 mM NaCl, 10 mM Tris-chloride, pH 7.4, half-life: 4 days, best conditions of storage Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: glutathione Rattus norvegicus ?
-
?
additional information not: cysteine Rattus norvegicus ?
-
?
S-adenosyl-L-methionine + a thiol high specificity for S-adenosyl-L-methionine Rattus norvegicus S-adenosyl-L-homocysteine + a thioether
-
?
2-mercaptoethanol + S-adenosyl-L-methionine
-
Rattus norvegicus S-methyl-2-mercaptoethanol + S-adenosyl-L-homocysteine
-
?
mercaptoacetic acid + S-adenosyl-L-methionine i.e. thioglycolic acid Rattus norvegicus S-methylmercaptoacetic acid + S-adenosyl-L-homocysteine
-
?
S-tubercidinyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-tubercidinyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
S-3-deazaadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-3-deazaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
S-N6-methyladenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-N6-methyladenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
S-8-azaadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-8-azaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
S-3'-deoxyadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-3'-deoxyadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
S-aristeromycinyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-aristeromycinyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9 S-methylation of 2-mercaptoethanol Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.9
-
30°C, absence of nucleotide substrate, half-life: less than 1 min Rattus norvegicus