Application | Comment | Organism |
---|---|---|
medicine | a cloned source of this enzyme should be useful in the identification of cellular substrates and for quantitation and localization of isoaspartyl sites in purified proteins, including recombinant proteins used as pharmaceuticals | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
PCR amplified cDNA cloned into vector pdeltablue and expressed in Escherichia coli | Rattus norvegicus |
General Stability | Organism |
---|---|
no stability problems during purification | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00089 | - |
S-adenosyl-L-methionine | native brain enzyme | Rattus norvegicus | |
0.0011 | - |
S-adenosyl-L-methionine | recombinant protein | Rattus norvegicus | |
0.0164 | - |
isoAspDSIP | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-isoaspartate | Escherichia coli | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Rattus norvegicus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Bos taurus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Escherichia coli | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.014 | - |
- |
Rattus norvegicus |
0.016 | 0.02 | gamma-globulin as methyl acceptor | Rattus norvegicus |
0.0168 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, can be stored in 10% glycerol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + gamma-globulin | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + gamma-globulin | - |
Bos taurus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + isoAspDSIP | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Escherichia coli | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Bos taurus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PIMT | - |
Rattus norvegicus |