Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | relative activity 70% of control | Oryza sativa | |
Ca2+ | relative activity 58% of control | Oryza sativa | |
Co2+ | strongly inhibits, relative activity 7% of control | Oryza sativa | |
Cu2+ | relative activity 0% of control | Oryza sativa | |
EDTA | relative activity 77% of control | Oryza sativa | |
Hg2+ | relative activity 0% of control | Oryza sativa | |
Mg2+ | relative activity 64% of control | Oryza sativa | |
Mn2+ | relative activity 24% of control | Oryza sativa | |
additional information | no increase in the expression of OsCOMT1 mRNA detected in CuCl2-, jasmonic acid and UV treated leaves | Oryza sativa | |
Zn2+ | relative activity 9% of control | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
S-adenosyl-L-methionine | when caffeic acid is supplied at a constant concentration of 1 mM | Oryza sativa | |
0.069 | - |
caffeic acid | when S-adenosyl-L-methionine is supplied at a constant concentration of 1 mM | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
purified enzyme, SDS-PAGE | Oryza sativa |
42300 | - |
sequence analysis | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by His-Bind column chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aerial part | - |
Oryza sativa | - |
leaf | - |
Oryza sativa | - |
root | - |
Oryza sativa | - |
Storage Stability | Organism |
---|---|
-80°C, Tris-HCl buffer, 10% glycerol and 2-mercaptoethanol, pH 8.0 | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eriodictyol | best substrate, 131% relative activity | Oryza sativa | ? | - |
? | |
luteolin | 88% relative activity | Oryza sativa | ? | - |
? | |
additional information | no acitivity with p-coumaric acid flavanones, naringenin, homoeriodictyol, hesperetin flavonols, kaempferol, isorhametin flavones, apigenin and chrysoeriol | Oryza sativa | ? | - |
? | |
myricetin | 75% relative activity | Oryza sativa | ? | - |
? | |
quercetin | 71% relative activity | Oryza sativa | ? | - |
? | |
S-adenosyl-L-methionine + caffeic acid | 100% relative activity | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caffeic acid 3-O-methyltransferase | - |
Oryza sativa |
OsCOMT1 | previously reported as NOMT | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Oryza sativa |