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Literature summary for 2.1.1.382 extracted from

  • Grimm, C.; Lazzarotto, M.; Pompei, S.; Schichler, J.; Richter, N.; Farnberger, J.; Fuchs, M.; Kroutil, W.
    Oxygen-free regioselective biocatalytic demethylation of methyl-phenyl ethers via methyltransfer employing veratrol-O-demethylase (2020), ACS Catal., 10, 10375-10380 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.382

Application

Application Comment Organism
synthesis the demethylation by VdmB transforms a variety of aryl methyl ethers with two functional methoxy moieties either in 1,2-position or in 1,3-position. Biocatalytic reactions enable the regioselective monodemethylation of substituted 3,4-dimethoxy phenol as well as the monodemethylation of 1,3,5-trimethoxybenzene. The VdmB is also applied for the regioselective demethylation of natural compounds such as papaverine and rac-yatein. Best results are obtained in presence of orcinol as final methyl acceptor Acetobacterium dehalogenans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ highest conversion rates are obtained in presence of 0.02 mM Zn2+ Acetobacterium dehalogenans

Organism

Organism UniProt Comment Textmining
Acetobacterium dehalogenans Q6W001
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3,4-dimethoxyphenyl)methanol + a [Co(I) methoxylated corrinoid protein dhaf4611] dhaf4611, i.e. carrier protein from Desulfitobacterium hafniense Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + 4-(hydroxymethyl)-2-methoxyphenol + 5-(hydroxymethyl)-2-methoxyphenol
-
 ?
1,2-dimethoxy-4-methylbenzol + a [Co(I) methoxylated corrinoid protein dhaf4611] dhaf4611, i.e. carrier protein from Desulfitobacterium hafniense Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + 2-methoxy-4-methylphenol + 2-methoxy-5-methylphenol
-
 ?
2,3-dimethoxynaphthalene + a [Co(I) methoxylated corrinoid protein dhaf4611]
-
 Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + 3-methoxynaphthalen-2-ol + naphthalen-2,3-diol
-
 ?
3,4-dimethoxybenzaldehyde + a [Co(I) methoxylated corrinoid protein dhaf4611] dhaf4611, i.e. carrier protein from Desulfitobacterium hafniense Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + 4-hydroxy-3-methoxy-benzaldehyde + 2-hydroxy-3-methoxy-benzaldehyde
-
 ?
3,4-dimethoxyphenol + a [Co(I) methoxylated corrinoid protein dhaf4611] dhaf4611, i.e. carrier protein from Desulfitobacterium hafniense Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + 4-methoxybenzene-1,3-diol
-
 ?
veratrol + a [Co(I) methoxylated corrinoid protein dhaf4611] dhaf4611, i.e. carrier protein from Desulfitobacterium hafniense Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein dhaf4611] + guaiacol
-
 ?
veratrol + a [Co(I) methoxylated corrinoid protein VdmA]
-
 Acetobacterium dehalogenans a [methyl-Co(III) methoxylated corrinoid protein VdmA] + guaiacol
-
 ?

Synonyms

Synonyms Comment Organism
vdmB
-
 Acetobacterium dehalogenans
veratrol-O-demethylase
-
 Acetobacterium dehalogenans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Acetobacterium dehalogenans

General Information

General Information Comment Organism
metabolism oxygen-independent demethylation via methyl transfer is mediated by cobalamin-dependent veratrol-O-demethylase VdmB Acetobacterium dehalogenans