Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DNA(cytosine-S)-methyltransferase 3-like protein | i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of Dnmt3B by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding | Mus musculus | |
DNA(cytosine-S)-methyltransferase 3-like protein | i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of the Dnmt3A by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Complex formation between Dnmt3A and Dnmt3L accelerates DNA binding by Dnmt3A 20fold and lowers its Km for DNA. Interaction of Dnmt3L with Dnmt3A increases the binding of AdoMet to Dnmt3A, and lowers the Km-value of Dnmt3A for AdoMet. Interaction of Dnmt3A and Dnmt3L is transient, and after DNA binding to Dnmt3A, Dnmt3L dissociates from the complex. Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
DNA | enzyme Dnmt3A, in presence of Dnmt3L | Mus musculus | |
0.00076 | - |
DNA | enzyme Dnmt3A, in absence of Dnmt3L | Mus musculus | |
0.00096 | - |
S-adenosyl-L-methionine | enzyme Dnmt3A, in presence of Dnmt3L | Mus musculus | |
0.00255 | - |
S-adenosyl-L-methionine | enzyme Dnmt3A, in absence of Dnmt3L | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O88508 | recombinant, enzyme Dnmt3A | - |
Mus musculus | O88509 | recombinant, enzyme Dnmt3B | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA | - |
Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-methionine | enzyme Dnmt3A, in absence of Dnmt3L | Mus musculus | |
0.01 | - |
DNA | enzyme Dnmt3A, in absence of Dnmt3L | Mus musculus | |
0.013 | - |
S-adenosyl-L-methionine | enzyme Dnmt3A, in absence of Dnmt3L | Mus musculus | |
0.013 | - |
DNA | enzyme Dnmt3A, in presence of Dnmt3L | Mus musculus |