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Literature summary for 2.1.1.35 extracted from

  • Kealey, J.T.; Gu, X.; Santi, D.V.
    Enzymatic mechanism of tRNA (m5U54)methyltransferase (1994), Biochimie, 76, 1133-1142.
    View publication on PubMed
Search for more literature for 2.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
trmA gene encodes enzyme, gene is essential for the organism Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-Fluorouracil substituted tRNA potent inhibitor Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
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Organism

Organism UniProt Comment Textmining
Escherichia coli
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Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA steric course of methyl transfer Escherichia coli
a
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA single SN2 displacement mechanism Escherichia coli
a
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA detailed chemical and enzymatic mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information detailed mechanism, Cys-324 is the nucleophilic catalyst of enzyme, involves SN2 attack of C5 of U54 on the methyl group of AdoMet Escherichia coli additional information
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additional information up to 50% of enzyme is covalently bound to various tRNA species, including several tRNAs at the 3’ end of 16S rRNA Escherichia coli additional information
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additional information enzyme also catalyzes an S-adenosyl-L-methionine-independent exchange reaction between [5-3H]-Urd-labeled substrate tRNA and protons of water at 1% of the normal methylation reaction rate, mechanism Escherichia coli additional information
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S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop ?
S-adenosyl-L-methionine + uridine54 in tRNA tRNA recognition Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop ?
S-adenosyl-L-methionine + uridine54 in tRNA enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
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 ?

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine uses S-adenosyl-L-methionine as cofactor Escherichia coli