BRENDA - Enzyme Database show
show all sequences of 2.1.1.344

OlsG (Sinac_1600) Is an Ornithine Lipid N-Methyltransferase from the Planctomycete Singulisphaera acidiphila

Escobedo-Hinojosa, W.I.; Vences-Guzman, M.A.; Schubotz, F.; Sandoval-Calderon, M.; Summons, R.E.; Lopez-Lara, I.M.; Geiger, O.; Sohlenkamp, C.; J. Biol. Chem. 290, 15102-15111 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
-
Singulisphaera acidiphila
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Singulisphaera acidiphila
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0377
-
an ornithine lipid
at pH 8.0 and 28°C
Singulisphaera acidiphila
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + an ornithine lipid
Singulisphaera acidiphila
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
?
S-adenosyl-L-methionine + an ornithine lipid
Singulisphaera acidiphila DSM 18658T
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Singulisphaera acidiphila
L0D9B6
-
-
Singulisphaera acidiphila DSM 18658T
L0D9B6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + an ornithine lipid
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
742872
Singulisphaera acidiphila
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
-
?
S-adenosyl-L-methionine + an ornithine lipid
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
742872
Singulisphaera acidiphila DSM 18658T
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Singulisphaera acidiphila
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on, 1 mM used in assay conditions
Singulisphaera acidiphila
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
-
Singulisphaera acidiphila
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Singulisphaera acidiphila
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on, 1 mM used in assay conditions
Singulisphaera acidiphila
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0377
-
an ornithine lipid
at pH 8.0 and 28°C
Singulisphaera acidiphila
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + an ornithine lipid
Singulisphaera acidiphila
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
?
S-adenosyl-L-methionine + an ornithine lipid
Singulisphaera acidiphila DSM 18658T
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + an ornithine lipid
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
742872
Singulisphaera acidiphila
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
-
?
S-adenosyl-L-methionine + an ornithine lipid
the enzyme is responsible for the 3-fold methylation of the terminal delta-nitrogen of ornithine lipids
742872
Singulisphaera acidiphila DSM 18658T
S-adenosyl-L-homocysteine + an N,N,N-trimethylornithine lipid
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Singulisphaera acidiphila
Other publictions for EC 2.1.1.344
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742872
Escobedo-Hinojosa
OlsG (Sinac_1600) Is an Ornit ...
Singulisphaera acidiphila, Singulisphaera acidiphila DSM 18658T
J. Biol. Chem.
290
15102-15111
2015
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