Literature summary for 2.1.1.327 extracted from

Parsons, J.F.; Greenhagen, B.T.; Shi, K.; Calabrese, K.; Robinson, H.; Ladner, J.E.
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa (2007), Biochemistry, 46, 1821-1828.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
1.8 A crystal structure of PhzM. PhzM is a dimer in solution. The polypeptide folds into three domains. The C-terminal domain exhibits the alpha/beta-hydrolase fold typical of small molecule methyltransferases. Two smaller N-terminal domains form much of the dimer interface. PhzM is most similar to the plant O-methyltransferases that are characterized by an unusual intertwined dimer interface. The structure of PhzM contains no ligands and the active site is open and solvent exposed	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

1.8 A crystal structure of PhzM. PhzM is a dimer in solution. The polypeptide folds into three domains. The C-terminal domain exhibits the alpha/beta-hydrolase fold typical of small molecule methyltransferases. Two smaller N-terminal domains form much of the dimer interface. PhzM is most similar to the plant O-methyltransferases that are characterized by an unusual intertwined dimer interface. The structure of PhzM contains no ligands and the active site is open and solvent exposed

Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	-	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HWH2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	purified PhzM alone has little or no ability to methylate phenzine-1-carboxylic acid. When the putative hydroxylase PhzS, EC 1.14.13.218, is included, pyocyanin is readily produced	Pseudomonas aeruginosa	?	-	?
S-adenosyl-L-methionine + phenazine-1-carboxylate	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + 5-methyl-phenazine-1-carboxylate	-	?

Synonyms

Synonyms	Comment	Organism
phzM	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	phenazine-1-carboxylate	presence of hydroxylase PhzS, pH 7.8, 22°C	Pseudomonas aeruginosa