Literature summary for 2.1.1.297 extracted from

Kemp, G.; Kudva, R.; de la Rosa, A.; von Heijne, G.
Force-profile analysis of the cotranslational folding of HemK and filamin domains comparison of biochemical and biophysical folding assays (2019), J. Mol. Biol., 431, 1308-1314 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene HEMK1, recombinant expression of isolated N-terminal domain and of truncated mutants in Escherichia coli, cotranslational folding of the HemK N-terminal domain	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L27A/L28A	site-directed mutagenesis	Homo sapiens
L28A	site-directed mutagenesis, the L28A mutation reduces the in vitro denaturation temperature of HemK-N-terminal domain from 50°C to 30°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y5R4	-	-

Synonyms

Synonyms	Comment	Organism
HemK	-	Homo sapiens
Hemk1	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	the cotranslational folding of two small protein domains of different folds - the alpha-helical N-terminal domain of HemK and the beta-rich FLN5 filamin domain - by measuring the force that the folding protein exerts on the nascent chain when located in different parts of the ribosome exit tunnel (force-profile analysis, or FPA), allowing us to compare FPA to three other techniques currently used to study cotranslational folding: real-time FRET, photoinduced electron transfer, and NMR, method evaluation, overview	Homo sapiens

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Release 2023.1 (January 2023)