BRENDA - Enzyme Database show
show all sequences of 2.1.1.274

An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense

Chen, F.; D'Auria, J.C.; Tholl, D.; Ross, J.R.; Gershenzon, J.; Noel, J.P.; Pichersky, E.; Plant J. 36, 577-588 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
alamethicin
treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission
Arabidopsis thaliana
insect herbivory
feeding by the larvae of the diamondback moth on rosette leaves of intact plants resulted in low methyl salicylate and very low methyl benzoate emission
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
Arabidopsis lyrata
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
salicylic acid
apparent Km-value, pH 7.7, 30°C
Arabidopsis thaliana
0.067
-
S-adenosyl-L-methionine
apparent Km-value, pH 7.7, 30°C
Arabidopsis thaliana
0.087
-
S-adenosyl-L-methionine
apparent Km-value, pH 7.7, 30°C
Arabidopsis lyrata
0.127
-
salicylic acid
apparent Km-value, pH 7.7, 30°C
Arabidopsis lyrata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43360
-
AlBSMT1 subunit, calculated from sequence
Arabidopsis lyrata
43363
-
2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence
Arabidopsis lyrata
43365
-
2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence
Arabidopsis thaliana
43370
-
AtBSMT1 subunit, calculated from sequence
Arabidopsis thaliana
44000
-
AlBSMT1 subunit mass by SDS–PAGE
Arabidopsis lyrata
44000
-
AtBSMT1 subunit mass by SDS–PAGE
Arabidopsis thaliana
91000
-
AlBSMT1 holoenzyme, estimated by size exclusion chromatography
Arabidopsis lyrata
91000
-
AtBSMT1 holoenzyme, estimated by size exclusion chromatography
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis lyrata
Q6XMI1
-
-
Arabidopsis thaliana
Q6XMI3
-
-
Purification (Commentary)
Commentary
Organism
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography
Arabidopsis lyrata
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers
Arabidopsis lyrata
-
flower
highest levels of AtBSMT1 transcript
Arabidopsis thaliana
-
leaf
detectable AlBSMT1 transcript levels
Arabidopsis lyrata
-
leaf
lower levels of AtBSMT1 transcript
Arabidopsis thaliana
-
additional information
little or no AtBSMT1 transcripts observed in roots or siliques of plants
Arabidopsis thaliana
-
stem
lower levels of AtBSMT1 transcript
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
723416
Arabidopsis thaliana
?
-
-
-
?
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
723416
Arabidopsis lyrata
?
-
-
-
?
additional information
in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid
723416
Arabidopsis lyrata
?
-
-
-
?
additional information
The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase
723416
Arabidopsis thaliana
?
-
-
-
?
additional information
The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase
723416
Arabidopsis lyrata
?
-
-
-
?
S-adenosyl-L-methionine + 3-hydroxybenzoic acid
54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + anthranilic acid
32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis lyrata
S-adenosyl-L-homocysteine + methyl anthranilate
-
-
-
?
S-adenosyl-L-methionine + anthranilic acid
35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl anthranilate
-
-
-
?
S-adenosyl-L-methionine + salicylic acid
16% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis lyrata
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
S-adenosyl-L-methionine + salicylic acid
46% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence
Arabidopsis lyrata
homodimer
2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
salicylic acid
pH 7.7, 30°C
Arabidopsis lyrata
0.07
-
salicylic acid
pH 7.7, 30°C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Arabidopsis lyrata
7
8
-
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
alamethicin
treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission
Arabidopsis thaliana
insect herbivory
feeding by the larvae of the diamondback moth on rosette leaves of intact plants resulted in low methyl salicylate and very low methyl benzoate emission
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
Arabidopsis lyrata
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
salicylic acid
apparent Km-value, pH 7.7, 30°C
Arabidopsis thaliana
0.067
-
S-adenosyl-L-methionine
apparent Km-value, pH 7.7, 30°C
Arabidopsis thaliana
0.087
-
S-adenosyl-L-methionine
apparent Km-value, pH 7.7, 30°C
Arabidopsis lyrata
0.127
-
salicylic acid
apparent Km-value, pH 7.7, 30°C
Arabidopsis lyrata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43360
-
AlBSMT1 subunit, calculated from sequence
Arabidopsis lyrata
43363
-
2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence
Arabidopsis lyrata
43365
-
2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence
Arabidopsis thaliana
43370
-
AtBSMT1 subunit, calculated from sequence
Arabidopsis thaliana
44000
-
AlBSMT1 subunit mass by SDS–PAGE
Arabidopsis lyrata
44000
-
AtBSMT1 subunit mass by SDS–PAGE
Arabidopsis thaliana
91000
-
AlBSMT1 holoenzyme, estimated by size exclusion chromatography
Arabidopsis lyrata
91000
-
AtBSMT1 holoenzyme, estimated by size exclusion chromatography
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography
Arabidopsis lyrata
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers
Arabidopsis lyrata
-
flower
highest levels of AtBSMT1 transcript
Arabidopsis thaliana
-
leaf
detectable AlBSMT1 transcript levels
Arabidopsis lyrata
-
leaf
lower levels of AtBSMT1 transcript
Arabidopsis thaliana
-
additional information
little or no AtBSMT1 transcripts observed in roots or siliques of plants
Arabidopsis thaliana
-
stem
lower levels of AtBSMT1 transcript
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
723416
Arabidopsis thaliana
?
-
-
-
?
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
723416
Arabidopsis lyrata
?
-
-
-
?
additional information
in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid
723416
Arabidopsis lyrata
?
-
-
-
?
additional information
The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase
723416
Arabidopsis thaliana
?
-
-
-
?
additional information
The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase
723416
Arabidopsis lyrata
?
-
-
-
?
S-adenosyl-L-methionine + 3-hydroxybenzoic acid
54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + anthranilic acid
32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis lyrata
S-adenosyl-L-homocysteine + methyl anthranilate
-
-
-
?
S-adenosyl-L-methionine + anthranilic acid
35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl anthranilate
-
-
-
?
S-adenosyl-L-methionine + salicylic acid
16% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis lyrata
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
S-adenosyl-L-methionine + salicylic acid
46% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100%
723416
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence
Arabidopsis lyrata
homodimer
2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
salicylic acid
pH 7.7, 30°C
Arabidopsis lyrata
0.07
-
salicylic acid
pH 7.7, 30°C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Arabidopsis lyrata
7
8
-
Arabidopsis thaliana
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella
additional information
Arabidopsis thaliana
leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective; methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction
up
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella
additional information
Arabidopsis thaliana
leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective; methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.4
-
salicylic acid
pH 7.7, 30°C
Arabidopsis lyrata
4.3
-
salicylic acid
pH 7.7, 30°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.4
-
salicylic acid
pH 7.7, 30°C
Arabidopsis lyrata
4.3
-
salicylic acid
pH 7.7, 30°C
Arabidopsis thaliana
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
Lilium hybrid cultivar
Genet. Mol. Res.
14
14510-14521
2015
-
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
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-
1
1
-
-
-
-
-
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-
-
1
2
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
736696
Krol
Priming of seeds with methyl j ...
Solanum lycopersicum
J. Plant Physiol.
179
122-132
2015
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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2
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1
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1
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1
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1
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1
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2
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1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
736842
Ludwig-Mueller
A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
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1
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1
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2
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1
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1
1
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2
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1
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1
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
567-577
2014
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
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-
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1
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1
1
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1
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1
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1
-
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1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726129
Lin
Overexpression of a soybean sa ...
Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
-
-
1
-
-
-
6
1
-
2
-
5
-
5
-
-
-
-
-
1
-
-
5
-
1
1
-
-
1
1
-
-
-
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-
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-
1
-
-
-
-
-
6
-
1
-
2
-
5
-
-
-
-
-
1
-
-
5
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
725781
Yao
QM/MM free energy simulations ...
Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
-
-
-
-
-
-
-
-
-
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2
-
3
-
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2
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2
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2
-
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-
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-
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-
726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
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-
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-
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-
-
2
2
-
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-
726172
Tieman
Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
-
-
1
-
-
-
-
2
-
-
-
4
-
5
-
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-
-
4
-
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4
-
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2
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1
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2
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-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
2
-
-
-
-
1
1
1
1
-
-
726132
Kwon
-
Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
-
-
-
-
-
-
-
-
-
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1
-
1
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3
-
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1
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1
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3
-
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1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
-
-
1
1
5
-
-
1
-
-
1
-
-
4
-
-
1
-
-
-
-
-
6
1
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1
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1
-
1
5
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1
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1
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1
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-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
-
2
-
-
-
-
4
-
-
8
-
-
2
-
-
2
-
-
6
-
-
10
2
-
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-
2
2
-
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2
-
2
-
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-
-
-
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-
4
-
-
8
-
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-
2
-
6
-
-
10
2
-
-
-
2
2
-
-
-
2
-
-
2
2
2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
-
-
1
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S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
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9-16
1999
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