BRENDA - Enzyme Database
show all sequences of 2.1.1.274

S-Adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases

Ross, J.R.; Nam, K.H.; D'Auria, J.C.; Pichersky, E.; Arch. Biochem. Biophys. 367, 9-16 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
SAMT cDNA expressed with pET-11a/pET-28 vector in Escherichia coli which synthesizes a functional non-tagged/His-tagged SAMT protein
Clarkia breweri
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
Cu2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Fe2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Fe3+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Mg2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
Mn2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
additional information
5 mM Na+ and NH4+ have no effect on activity
Clarkia breweri
Zn2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
salicylate
app. Km-value of His-tagged protein, pH 7.5, 20C
Clarkia breweri
0.009
-
S-adenosyl-L-methionine
app. Km-value of nontagged and His-tagged protein, pH 7.5, 20C
Clarkia breweri
0.024
-
salicylate
app. Km-value of nontagged protein, pH 7.5, 20C
Clarkia breweri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
5 mM, stimulates SAMT activity by a factor of 2
Clarkia breweri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
monomer after Mono-Q chromatography
Clarkia breweri
40300
-
calculated from sequence
Clarkia breweri
74500
-
native protein
Clarkia breweri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + salicylate
Clarkia breweri
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Clarkia breweri
Q9SPV4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography
Clarkia breweri
Reaction
Reaction
Commentary
Organism
Reaction ID
S-adenosyl-L-methionine + salicylate = S-adenosyl-L-homocysteine + methyl salicylate
the enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase
Clarkia breweri
Source Tissue
Source Tissue
Commentary
Organism
Textmining
petal
highest SAMT activity compared to the levels of activity in all other floral parts or the vegetative tissue
Clarkia breweri
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000228
-
crude extract, pH 7.5, 20C
Clarkia breweri
0.00069
-
DE-53 DEAE chromatography, pH 7.5, 20C
Clarkia breweri
0.00087
-
HAP chromatography, pH 7.5, 20C
Clarkia breweri
0.02118
-
Mono-Q chromatography, pH 7.5, 20C
Clarkia breweri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
721412
Clarkia breweri
?
-
-
-
?
additional information
no activity with 4-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid and (+/-)jasmonic acid
721412
Clarkia breweri
?
-
-
-
?
S-adenosyl-L-methionine + 3-hydroxybenzoic acid
less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100%
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + cinnamic acid
less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100%
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl cinnamate
-
-
-
?
S-adenosyl-L-methionine + salicylate
-
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40300, calculated from sequence
Clarkia breweri
Synonyms
Synonyms
Commentary
Organism
S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase
-
Clarkia breweri
SAMT
-
Clarkia breweri
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
40
SAMT is 100% stable for 30 min at 20C and 80% stable for 30 min at 30C, but after 30 min incubation at 40C it completely loses activity
Clarkia breweri
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8
-
salicylate
nontagged protein, pH 7.5, 20C
Clarkia breweri
14
-
salicylate
His-tagged protein, pH 7.5, 20C
Clarkia breweri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Clarkia breweri
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
at pH 6.0 activity is 80% of optimal activity, at pH 8.0 it is 75%, and at pH 9 it is less than 50%
Clarkia breweri
Cloned(Commentary) (protein specific)
Commentary
Organism
SAMT cDNA expressed with pET-11a/pET-28 vector in Escherichia coli which synthesizes a functional non-tagged/His-tagged SAMT protein
Clarkia breweri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
Cu2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Fe2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Fe3+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
Mg2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
Mn2+
5 mM, mild inhibitory effect, less than 35% reduced activity
Clarkia breweri
additional information
5 mM Na+ and NH4+ have no effect on activity
Clarkia breweri
Zn2+
5 mM, strong inhibitory effect with 50100% inhibition
Clarkia breweri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
salicylate
app. Km-value of His-tagged protein, pH 7.5, 20C
Clarkia breweri
0.009
-
S-adenosyl-L-methionine
app. Km-value of nontagged and His-tagged protein, pH 7.5, 20C
Clarkia breweri
0.024
-
salicylate
app. Km-value of nontagged protein, pH 7.5, 20C
Clarkia breweri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
5 mM, stimulates SAMT activity by a factor of 2
Clarkia breweri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
monomer after Mono-Q chromatography
Clarkia breweri
40300
-
calculated from sequence
Clarkia breweri
74500
-
native protein
Clarkia breweri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + salicylate
Clarkia breweri
-
S-adenosyl-L-homocysteine + methyl salicylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography
Clarkia breweri
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
petal
highest SAMT activity compared to the levels of activity in all other floral parts or the vegetative tissue
Clarkia breweri
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000228
-
crude extract, pH 7.5, 20C
Clarkia breweri
0.00069
-
DE-53 DEAE chromatography, pH 7.5, 20C
Clarkia breweri
0.00087
-
HAP chromatography, pH 7.5, 20C
Clarkia breweri
0.02118
-
Mono-Q chromatography, pH 7.5, 20C
Clarkia breweri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme is also active with benzoic acid resulting in methyl benzoate formation
721412
Clarkia breweri
?
-
-
-
?
additional information
no activity with 4-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid and (+/-)jasmonic acid
721412
Clarkia breweri
?
-
-
-
?
S-adenosyl-L-methionine + 3-hydroxybenzoic acid
less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100%
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
-
-
?
S-adenosyl-L-methionine + cinnamic acid
less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100%
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl cinnamate
-
-
-
?
S-adenosyl-L-methionine + salicylate
-
721412
Clarkia breweri
S-adenosyl-L-homocysteine + methyl salicylate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40300, calculated from sequence
Clarkia breweri
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
40
SAMT is 100% stable for 30 min at 20C and 80% stable for 30 min at 30C, but after 30 min incubation at 40C it completely loses activity
Clarkia breweri
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8
-
salicylate
nontagged protein, pH 7.5, 20C
Clarkia breweri
14
-
salicylate
His-tagged protein, pH 7.5, 20C
Clarkia breweri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Clarkia breweri
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
at pH 6.0 activity is 80% of optimal activity, at pH 8.0 it is 75%, and at pH 9 it is less than 50%
Clarkia breweri
Other publictions for EC 2.1.1.274
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
Lilium hybrid cultivar
Genet. Mol. Res.
14
14510-14521
2015
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1
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1
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2
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736696
Krol
Priming of seeds with methyl j ...
Solanum lycopersicum
J. Plant Physiol.
179
122-132
2015
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1
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1
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1
1
1
1
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736842
Ludwig-Mueller
A novel methyltransferase from ...
Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
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1
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5
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1
1
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736841
Zhu
Salicylic acid and jasmonic ac ...
Nicotiana benthamiana
Mol. Plant Microbe Interact.
27
567-577
2014
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-
1
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1
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1
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6
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1
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1
1
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726129
Lin
Overexpression of a soybean sa ...
Glycine max
Plant Biotechnol. J.
11
1135-1145
2013
-
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1
-
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6
1
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2
-
5
-
5
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1
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5
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2
1
1
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1
1
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1
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6
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1
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5
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1
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1
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1
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1
1
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725781
Yao
QM/MM free energy simulations ...
Clarkia breweri
J. Phys. Chem. B
115
389-396
2011
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726013
Liu
Altering expression of benzoic ...
Arabidopsis thaliana
Mol. Plant Microbe Interact.
23
82-90
2010
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5
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2
2
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726172
Tieman
Functional analysis of a tomat ...
Solanum lycopersicum
Plant J.
62
113-123
2010
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1
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1
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726132
Kwon
-
Biotic and abiotic stresses in ...
Atropa belladonna
Plant Biotechnol.
26
207-215
2009
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1
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1
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723402
Zubieta
Structural basis for substrate ...
Clarkia breweri
Plant Cell
15
1704-1716
2003
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1
1
5
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1
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1
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1
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1
1
723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
-
2
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4
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8
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6
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10
2
2
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2
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10
2
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2
2
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2
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2
2
2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
-
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1
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1
6
2
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2
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4
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1
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4
1
1
3
1
2
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1
2
1
2
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1
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1
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6
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2
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2
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1
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4
1
1
3
1
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1
2
1
2
-
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-
721412
Ross
S-Adenosyl-L-methionine:salicy ...
Clarkia breweri
Arch. Biochem. Biophys.
367
9-16
1999
-
-
1
-
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-
8
3
-
1
3
1
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6
-
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1
1
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4
4
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5
1
2
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1
2
1
1
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1
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8
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3
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1
3
1
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1
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4
4
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5
1
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1
2
1
1
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