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show all sequences of 2.1.1.270

Inactivation of pea genes by RNAi supports the involvement of two similar O-methyltransferases in the biosynthesis of (+)-pisatin and of chiral intermediates with a configuration opposite that found in (+)-pisatin

Kaimoyo, E.; VanEtten, H.; Phytochemistry 69, 76-87 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
Pisum sativum
HMM2 is involved primarily in the biosynthesis of (+)-pisatin
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Pisum sativum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in Pisum sativum, there are two highly similar hydroxymaackiain 3-O-methyltransferases HMM, with HMM1 prefering 2,7,4'-trihydroxyisoflavanone as a substrate and HMM2 being the specific O-methyltransferase that methylates (+)-6a-hydroxymaackiain
706124
Pisum sativum
?
-
-
-
-
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
isoform HMM2, 6a-hydroxymaackiain is the preferred substrate
706124
Pisum sativum
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
HMM2 is involved primarily in the biosynthesis of (+)-pisatin
706124
Pisum sativum
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
Pisum sativum
HMM2 is involved primarily in the biosynthesis of (+)-pisatin
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in Pisum sativum, there are two highly similar hydroxymaackiain 3-O-methyltransferases HMM, with HMM1 prefering 2,7,4'-trihydroxyisoflavanone as a substrate and HMM2 being the specific O-methyltransferase that methylates (+)-6a-hydroxymaackiain
706124
Pisum sativum
?
-
-
-
-
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
isoform HMM2, 6a-hydroxymaackiain is the preferred substrate
706124
Pisum sativum
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
HMM2 is involved primarily in the biosynthesis of (+)-pisatin
706124
Pisum sativum
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
hairy roots containing RNAi constructs of HMM are deficient in (+)-pisatin biosynthesis, and accumulate 2,7,4'-trihydroxyisoflavanone, daidzein, isoformononetin, and liquiritigenin. Accumulation of compounds is consistent with the blockage of the synthesis of (+)-pisatin at the HI4'OMT catalyzed step resulting in the accumulation of liquiritigenin and 2,7,4'-trihydroxyisoflavanone and the diversion of the pathway to produce daidzein and isoformononetin
Pisum sativum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
hairy roots containing RNAi constructs of HMM are deficient in (+)-pisatin biosynthesis, and accumulate 2,7,4'-trihydroxyisoflavanone, daidzein, isoformononetin, and liquiritigenin. Accumulation of compounds is consistent with the blockage of the synthesis of (+)-pisatin at the HI4'OMT catalyzed step resulting in the accumulation of liquiritigenin and 2,7,4'-trihydroxyisoflavanone and the diversion of the pathway to produce daidzein and isoformononetin
Pisum sativum
Other publictions for EC 2.1.1.270
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711599
Fondevilla
Identification of genes differ ...
Pisum sativum, Pisum sativum P665
BMC Genomics
12
28
2011
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1
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1
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-
706124
Kaimoyo
Inactivation of pea genes by R ...
Pisum sativum
Phytochemistry
69
76-87
2008
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1
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1
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1
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1
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1
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1
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3
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1
1
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-
676377
Akashi
Catalytic specificity of pea O ...
Pisum sativum
Phytochemistry
67
2525-2530
2006
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1
1
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2
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1
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5
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713268
Liu
Blount, J.W.; Huhman, D.; Dixo ...
Medicago truncatula
Plant Cell
18
3656-3669
2006
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-
1
1
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1
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1
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1
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2
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1
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1
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1
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1
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2
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1
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1
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1
1
441447
He
Stress responses in alfalfa (M ...
Medicago sativa
Plant Mol. Biol.
36
43-54
1998
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1
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1
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