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Literature summary for 2.1.1.270 extracted from

  • He, X.Z.; Reddy, J.T.; Dixon, R.A.
    Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase (1998), Plant Mol. Biol., 36, 43-54.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Medicago sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE, recombinant enzyme Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Medicago sativa in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago sativa O24529
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation Medicago sativa ?
-
 ?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 Medicago sativa S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
S-adenosyl-L-methionine + (+)-maackiain at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 Medicago sativa S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE, recombinant enzyme Medicago sativa

Synonyms

Synonyms Comment Organism
IOMT8
-
 Medicago sativa

Expression

Organism Comment Expression
Medicago sativa IOMT8 transcripts are rapidly induced following elicitation up