Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ti(III) citrate | dependent on | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
gene mtqA | - |
Methanosarcina barkeri NaT1 | - |
gene mtqA | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography | Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:tetramethylammonium-grown cell | - |
Methanosarcina barkeri | - |
additional information | cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity | Methanosarcina barkeri | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri | ? | - |
? | |
additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri NaT1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
MtqA | - |
Methanosarcina barkeri |
tetramethylammonium:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
additional information | cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 | Methanosarcina barkeri |