Cloned (Comment) | Organism |
---|---|
gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha | Sorghum bicolor |
gene VrROMTsyn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha. Recombinantly expressed enzyme from grape produces very small amounts of methylated resveratrol derivatives in Escherichia coli | Vitis riparia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sorghum bicolor | |
Mg2+ | required | Vitis riparia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | Sorghum bicolor | i.e. pinostilbene | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | Vitis riparia | i.e. pinostilbene | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | Sorghum bicolor | - |
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | Vitis riparia | - |
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | A8QW53 | gene SbROMT3syn | - |
Vitis riparia | K7XQ68 | gene VrROMTsyn | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene | Sorghum bicolor | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene | Vitis riparia | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene | Sorghum bicolor | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene | Vitis riparia | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | - |
Sorghum bicolor | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | - |
Vitis riparia | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene | Sorghum bicolor | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? | |
S-adenosyl-L-methionine + trans-resveratrol | major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene | Vitis riparia | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
Synonyms | Comment | Organism |
---|---|---|
resveratrol O-methyltransferase | - |
Sorghum bicolor |
resveratrol O-methyltransferase | - |
Vitis riparia |
ROMT | - |
Sorghum bicolor |
ROMT | - |
Vitis riparia |
SbROMT3syn protein | - |
Sorghum bicolor |
VrROMTsyn protein | - |
Vitis riparia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sorghum bicolor |
30 | - |
assay at | Vitis riparia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sorghum bicolor |
7.5 | - |
assay at | Vitis riparia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Sorghum bicolor | |
S-adenosyl-L-methionine | - |
Vitis riparia |
General Information | Comment | Organism |
---|---|---|
metabolism | biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview | Sorghum bicolor |
metabolism | biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview | Vitis riparia |