Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces venezuelae |
General Stability | Organism |
---|---|
inclusion of 0.1 mM S-adenosyl-L-methionine, 0.1 mM dithiothreitol, and 10% glycerol in in buffers is crucial to prevent TylM1 from precipitating during the purification | Streptomyces venezuelae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2766 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae | |
0.3074 | - |
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Streptomyces venezuelae |
47400 | - |
gel filtration | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose | Streptomyces venezuelae | the enzyme is involved in the biosynthesis of desosamine | 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | Q9ZGH6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | the kcat/KM for dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose is about 20fold lower than kcat/Km for the natural substrated TDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose | Streptomyces venezuelae | 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose | - |
? | |
2 S-adenosyl-L-methionine + dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose | the enzyme is involved in the biosynthesis of desosamine | Streptomyces venezuelae | 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | - |
? | |
2 S-adenosyl-L-methionine + TDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose | i.e. TDP-alpha-D-desosamine. When the reaction is quenched at an earlier time, the monomethylated species can also be detected | Streptomyces venezuelae | 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28000, SDS-PAGE | Streptomyces venezuelae |
homodimer | 2 * 28110, calculated from sequence | Streptomyces venezuelae |
Synonyms | Comment | Organism |
---|---|---|
DesVI | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Streptomyces venezuelae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae | |
1.533 | - |
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces venezuelae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces venezuelae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the N,N-dimethylation step in the biosynthesis of desosamine | Streptomyces venezuelae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae | |
5 | - |
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose | pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine | Streptomyces venezuelae |