Cloned (Comment) | Organism |
---|---|
recombinant expression of HA-tagged enzyme LCMT1 in Neuro-2a cells, coepression with Myc-tagged PME-1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of LCMT1-R71DELTA, a catalytically inactive mutant of LCMT1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | LCMT1 and methylated phosphatase 2A protein are concentrated in rafts, whereas demethylated phosphatase 2A protein and small amounts of PME-1 are preferentially distributed in non-raft membrane microdomains | Homo sapiens | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | Homo sapiens | - |
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
neuroblastoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | - |
Homo sapiens | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | absolut specific methylation of Leu309 in the C subunit of phosphatase 2A protein, no activity with Leu309DELTA PPA2 mutant | Homo sapiens | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LCMT1 | - |
Homo sapiens |
leucine carboxyl methyltransferase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | reduced methylation of phosphatase 2A protein through enzyme LCMT1, a major Tau phosphatase, occurs in Alzheimer disease. Altered phosphatase 2A protein and Tau membrane distribution can promote neuronal dysfunction and phospho-Tau pathology in Alzheimer disease. Methylation-incompetent L309DELTA mutant of PP2A C subunit is excluded from membrane rafts. Expression of the inactive LCMT1 mutant inhibits the accumulation of dephosphorylated and total Tau at the plasma membrane, whereas concomitantly enhancing Tau phosphorylation at the Ser422 phospho-epitope. Partial knockdown of LCMT1 in Neuro2a cells is associated with a 42-45% decrease in total amounts of membrane-associated Tau pools relative to controls | Homo sapiens |
physiological function | leucine carboxyl methyltransferase 1-dependent methylation regulates the association of protein phosphatase 2A and Tau protein with plasma membrane microdomains in neuroblastoma cells | Homo sapiens |