Cloned (Comment) | Organism |
---|---|
gene Os12g0240900, DNA and amino acid sequence determination and analysis, expression of GST-tagged NOMT in Escherichia coli strain Rosetta II (DE3) | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview | Oryza sativa | |
0.0019 | - |
(2S)-naringenin | pH 8.5, 30°C, purified recombinant GST-tagged NOMT | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (2S)-naringenin | Oryza sativa | - |
S-adenosyl-L-homocysteine + (2S)-sakuranetin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | I2FFE9 | var. japonica and cv. Nipponbare, gene Os12g0240900 | - |
Purification (Comment) | Organism |
---|---|
purification of native enzyme 400fold from caffeic acid 3-O-methyltransferase-deficient oscomt1 mutant rice plant leaves induced with UV-light by ammonium sulfate fractionation and adenosine affinity chromatography, recombinant GST-tagged NOMT from Escherichia coli strain Rosetta II (DE3) by glutathione affinity chromatography and ultrafiltration | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0036 | - |
purified NOMT, pH 8.5, 28°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is also active on kaempferol, apigenin, luteolin, liquiritigenin, and quercetin | Oryza sativa | ? | - |
? | |
S-adenosyl-L-methionine + (2S)-naringenin | - |
Oryza sativa | S-adenosyl-L-homocysteine + (2S)-sakuranetin | - |
? | |
S-adenosyl-L-methionine + (2S)-naringenin | - |
Oryza sativa | S-adenosyl-L-homocysteine + (2S)-sakuranetin | mass spectrometric and chiral analysis of sakuranetin product | ? |
Synonyms | Comment | Organism |
---|---|---|
NOMT | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 30 | assay at | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
(2S)-naringenin | pH 8.5, 30°C, purified recombinant GST-tagged NOMT | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | enzyme expression is inducible by by ultraviolet irradiation, CuCl2 treatment, jasmonic acid treatment, and infection by phytopathogens, e.g. fungus Magnaporthe oryzae | up |
General Information | Comment | Organism |
---|---|---|
metabolism | naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice | Oryza sativa |
additional information | sakuranetin has anti-inflammatory activity, anti-mutagenic activity, anti-pathogenic activities against Helicobacter pylori, Leishmania, and Trypanosoma and contributes to the maintenance of glucose homeostasis in animals | Oryza sativa |
physiological function | naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of major flavonoid phytoalexin sakuranetin in rice, sakuranetin is a useful compound as a plant antibiotic and a potential pharmaceutical agent | Oryza sativa |