Literature summary for 2.1.1.22 extracted from

Liu, X.; Wu, J.; Sun, Y.; Xie, W.
Substrate recognition mechanism of the putative yeast carnosine N-methyltransferase (2017), ACS Chem. Biol., 12, 2164-2171 .

Search for more literature for 2.1.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
-	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure in complex with SAH. At the cofactor-binding site, SAH forms an extensive interaction network with the enzyme. Two disulfide bridges might be functionally important to the enzyme	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
C123A	mutation in disulfide bridge, displays strongly enhanced activity compared to that of wild-type	Saccharomyces cerevisiae
C238A	mutation in disulfide bridge, significantly reduces the activity	Saccharomyces cerevisiae
D278A/T279A	mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
E167A	mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
H309A	about 40% of wild-type activity	Saccharomyces cerevisiae
L307F	activity similar to wild-type	Saccharomyces cerevisiae
N274A	substantial reduction in expression level. Mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
Q108A	mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
R111A	mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
S104A	activity similar to wild-type	Saccharomyces cerevisiae
S234A	mutant barely displays any affinity to SAM	Saccharomyces cerevisiae
Y286A	about 10fold lower affinity to SAM than wild-type	Saccharomyces cerevisiae
Y371A	substantial reduction in expression level, mutant barely displays any affinity to SAM	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P53934	-	-
Saccharomyces cerevisiae ATCC 204508	P53934	-	-

Synonyms

Synonyms	Comment	Organism
YNL092W	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)