Literature summary for 2.1.1.207 extracted from

Aubee, J.I.; Olu, M.; Thompson, K.M.
TrmL and TusA are necessary for rpoS and MiaA is required for hfq expression in Escherichia coli (2017), Biomolecules, 7, 39 .

Search for more literature for 2.1.1.207

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of trmL+ and trmL- PBAD-rpoS990-lacZ translational fusions (in rssB- backgrounds) cells, executed for PBAD-rpoS990-lacZ translational fusions with rpoS UUA-Leu CUX-Leu mutations (leu1)-strains KMT36002 and KMT36010, for rpoS UUG-Leu to CUX-leu mutation (leu2)-strains KMT37002 and KMT37010, and for rpoS with both UUA-Leu to CUX-Leu and UUG-Leu to CUX-Leu mutations (leu*3)-strains KMT33001 and KMT33013, overview. Comparison of expression of PBAD-rpoS990-lacZ translational fusions carrying wild-type UUX leucine codons with derivatives in which UUX codons are changed to CUX codons, in the presence and absence of TrmL or TusA. The absence of these proteins, and therefore presumably the modifications they catalyze, both abolish PBAD-rpoS990-lacZ translation activity. UUX-Leu to CUX-Leu codon mutations in rpoS suppress the trmL requirement for PBAD-rpoS990-lacZ expression	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu	Escherichia coli	-	S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu	-	?
S-adenosyl-L-methionine + cytidine34 in tRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AGJ7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the C/U34m modification occurs in leucine tRNACmAA and tRNAcmnm5UmAA. Both of these leucine tRNA isoacceptors recognize UUA-Leu and UUG-Leu codons, and also carry the i6A37 tRNA modification that assists in the optimal decoding of the Leu codons during rpoS translation	Escherichia coli	?	-	-
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu	-	Escherichia coli	S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu	-	?
S-adenosyl-L-methionine + cytidine34 in tRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA	-	?

Synonyms

Synonyms	Comment	Organism
Trml	-	Escherichia coli
tRNA (cytidine/uridine-2'O)-ribose methyltransferase L	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	the defect in RpoS translation in the absence of i6A37 prenyl transferase (MiaA) is due to the inability to add the C/U34m modification to UUX-Leu tRNAs	Escherichia coli
metabolism	role of several tRNA modifications on rpoS or hfq expression: 2'-O-methylation of cytidine or uridine, at the wobble position (C/Um), 2-thiouridine at the wobble position (s2U), as well as isopentenyl adenosine 37(i6A37), overview	Escherichia coli
physiological function	the addition of the 2'-O-methylcytidine/uridine 34 (C/U34m) tRNA modification by tRNA (cytidine/uridine-2'O)-ribose methyltransferase L (TrmL) in Escherichia coli requires the presence of the N6-isopentenyl adenosine 37 (i6A37). The C/U34m and s2U34 tRNA modifications are necessary for full proper rpoS translation. Gene trmL is necessary for leucine decoding during RpoS expression. The mechanism of action of the i6A37 tRNA modification on RpoS expression is, at least in part, promotion of efficient UUX-leucine decoding. Possibly the TrmL-catalyzed C/U34m tRNA modification is dispensable for hfq translation, while the MiaA catalyzed i6A37 is necessary for efficient hfq translation	Escherichia coli

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Release 2023.1 (January 2023)